Downregulation of TAF9B by miR-7-5p Inhibits the Progression of Osteosarcoma.

Abstract

BACKGROUND:

Osteosarcoma (OS) is a malignant bone tumor with high metastatic potential. As a regulatory factor of apoptosis, TATA-box binding protein (TBP) associated factor 9B (TAF9B) is rarely studied in tumors.

METHODS:

We investigated the role and mechanism of TAF9B in OS cells by overexpression and knockdown. CCK8, colony formation, transwell, and flow cytometry analysis were performed to detect proliferation, migration, invasion, and apoptosis.

RESULTS:

TAF9B overexpression promotes the proliferation, migration, and invasion of OS cells, while TAF9B knockdown gives the opposite result. TAF9B inhibits apoptosis by upregulating Bcl-2 and downregulating Bax and Cleaved-caspase-3. Through starBase analysis, it was found that miR-7-5p can bind to the 3'UTR region of TAF9B, which is further confirmed by the dual luciferase reporter system assay. MiR-7-5p downregulates the expression of TAF9B in MG63 and U2OS cells. The proliferation and invasion of OS cells are inhibited after miR-7-5p mimics transfection and are promoted after miR-7-5p inhibitor transfection. TAF9B rescues the inhibitory effect of miR-7-5p on OS cells. TAF9B also activates the AKT/mTOR signaling pathway.

CONCLUSION:

According to our results, miR-7-5p inhibits the translation of TAF9B and then suppresses growth and metastasis through the AKT/mTOR signaling pathway in OS cells, thereby indicating the potential value of miR-7-5p and TAF9B as therapeutic targets for human OS.

• Authors: Wanli Gu, Peng Chen, Peng Ren, Yanhai Wang, Xiaobing Li, Mingzhi Gong
• Journal: OncoTargets and therapy (Onco Targets Ther) Vol. 14 Pg. 2917-2927 ( 2021) ISSN: 1178-6930 [Print] New Zealand
• PMID: 33958878 (Publication Type: Journal Article)
• Copyright: © 2021 Gu et al.