Rutin restrains the growth and metastasis of mouse breast cancer cells by regulating the microRNA-129-1-3p-mediated calcium signaling pathway.

Abstract

Breast cancer is a common malignancy that is highly lethal. Due to the poor prognosis, more effective and efficient treatment methods are urgently needed. Rutin (RUT) is a traditional Chinese medicine reported to have a variety of pharmacological properties, including anticancer properties. However, the effects of RUT on breast cancer and its underlying molecular mechanism of action remain unclear. In the present study, we observed a significant downregulation of microRNA (miR)-129-1-3p in mouse breast cancer cells (4T1) compared with the expression in mouse normal breast epithelial cells (HC11). We also found that RUT could increase the expression of miR-129-1-3p in 4T1 cells and suppress cell proliferation. To elucidate the molecular mechanism of action of RUT, miR-129-1-3p mimics and its inhibitor were transfected into 4T1 cells. miR-129-1-3p overexpression could inhibit the proliferation, invasion, migration, and calcium overload of mouse breast cancer cells and also enhance apoptosis, whereas miR-129-1-3p knockdown had the opposite effects. Taken together, cell-based experiments indicated that RUT restrains the growth of mouse breast cancer cells by regulating the miR-129-1-3p/Ca2+ signaling pathway. This study also revealed the inhibitory effect of RUT on breast cancer cells at the noncoding RNA level and provided a theoretical foundation for the application of RUT as a drug to inhibit tumor growth.

• Authors: Qi Li, Dongsheng Xu, Zehui Gu, Tengteng Li, Peng Huang, Liqun Ren
• Journal: Journal of biochemical and molecular toxicology (J Biochem Mol Toxicol) Vol. 35 Issue 7 Pg. e22794 (Jul 2021) ISSN: 1099-0461 [Electronic] United States
• PMID: 33913213 (Publication Type: Journal Article)
• Copyright: © 2021 Wiley Periodicals LLC.

Chemical References

• MIRN129 microRNA, mouse
• MicroRNAs
• RNA, Neoplasm
• Rutin

Topics

• Animals
• Calcium Signaling (drug effects)
• Cell Line
• Female
• Mammary Neoplasms, Animal (genetics, metabolism, pathology)
• Mice
• MicroRNAs (genetics, metabolism)
• Neoplasm Metastasis
• RNA, Neoplasm (genetics, metabolism)
• Rutin (pharmacology)