Cross-priming amplification targeting the 18S rRNA gene for the rapid diagnosis of Babesia bovis infection.

Abstract

Babesia bovis is a known causative agent of bovine babesiosis and is widely distributed across China. Rapid detection and accurate identification of B. bovis is essential for follow-up management and epidemiological investigations. In this study, a cross-priming amplification combined with vertical flow (CPA-VF) assay was developed. The detection limit of the CPA-VF assay targeting the 18S rRNA gene was 320 fg per reaction at 61 °C for 60 min. No cross-reactions were observed with other piroplasms infective to cattle. Furthermore, 36 blood samples from experimentally-infected animals were accurately assessed using the CPA-VF assay. The performance of the CPA-VF assay was compared with the results of conventional PCR for 219 blood samples from the field. Our results demonstrate that the CPA-VF assay is a practical and effective diagnostic tool for bovine babesiosis caused by B. bovis infection.

• Authors: Jinming Wang, Shandian Gao, Jifei Yang, Junlong Liu, Youquan Li, Jianxun Luo, Guiquan Guan, Hong Yin
• Journal: Ticks and tick-borne diseases (Ticks Tick Borne Dis) Vol. 12 Issue 4 Pg. 101713 (07 2021) ISSN: 1877-9603 [Electronic] Netherlands
• PMID: 33827035 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier GmbH. All rights reserved.

Chemical References

• RNA, Protozoan
• RNA, Ribosomal, 18S

Topics

• Animals
• Babesia bovis (isolation & purification)
• Babesiosis (diagnosis, parasitology)
• Cattle
• Cattle Diseases (diagnosis, parasitology)
• Cross-Priming
• Nucleic Acid Amplification Techniques (methods, veterinary)
• RNA, Protozoan (analysis)
• RNA, Ribosomal, 18S (analysis)
• Sensitivity and Specificity