The influence of coconut oil on the growth, immune, and antioxidative responses and the intestinal digestive enzymes and histomorphometry features of Nile tilapia (Oreochromis niloticus).

Abstract

The trials of finding non-conventional and alternative aquafeed ingredients are increasing. In this sense, this study evaluated the influence of coconut oil on the growth, feed utilization, immune, and antioxidative responses of Nile tilapia. Five test diets were formulated by mixing coconut oil with the other ingredients at 0, 1, 2, 3, and 4% of the total ration and presented for tilapia for 60 successive days. The final weight, SGR, weight gain (WG), and feed intake were superior in fish delivered 2% of coconut oil (P < 0.05). Concurrently, fish that received 2% coconut oil had lower FCR and higher PER than fish of the control and 4% groups (P < 0.05). Higher lipase activity was observed in fish of 2% and 3% levels than the remaining groups (P < 0.05). Besides, the amylase and protease activities of fish in 1%, 2%, and 3% groups were higher than the 0% level (P < 0.05). The total blood cholesterol, RBCs, and PCV showed higher values in Nile tilapia fed 2% and 3% coconut oil (P < 0.05). The lysozyme and phagocytic activities were higher in fish fed 2% and 3% levels than the control (P < 0.05), while the phagocytic index in 2% and 3% levels was higher than 0% and 4% levels. Furthermore, SOD and CAT were higher in fish fed 1%, 2%, and 3% than fish fed 0% and 4% levels while GSH was higher in fish of 1%, 2%, and 3% than fish fed 0% level (P < 0.05). However, the MDA level was markedly lower in fish fed 25, 3%, and 4% coconut oil than the 0% level (P < 0.05). The intestine's histological structure in all groups appeared normal, forming of intestinal villi projecting from the intestinal wall. Also, the structure of the hepatopancreas had a normal architecture in all groups. To sum up, the inclusion of coconut oil at 2 to 3% is recommended as a replacer for fish oil in Nile tilapia diets.

• Authors: Mahmoud A O Dawood, Mohamed F Ali, Asem A Amer, Mahmoud S Gewaily, Mona M Mahmoud, Mohamed Alkafafy, Doaa H Assar, Ali A Soliman, Hien Van Doan
• Journal: Fish physiology and biochemistry (Fish Physiol Biochem) Vol. 47 Issue 4 Pg. 869-880 (Aug 2021) ISSN: 1573-5168 [Electronic] Netherlands
• PMID: 33770301 (Publication Type: Clinical Trial, Veterinary, Journal Article)
• Copyright: © 2021. The Author(s), under exclusive licence to Springer Nature B.V.

Chemical References

• Antioxidants
• Lipase
• Amylases
• Peptide Hydrolases
• Coconut Oil

Topics

• Amylases (metabolism)
• Animals
• Antioxidants
• Aquaculture (methods)
• Cichlids (anatomy & histology, growth & development, immunology, metabolism)
• Coconut Oil (pharmacology)
• Dietary Supplements
• Hepatopancreas (anatomy & histology)
• Intestines (anatomy & histology, enzymology)
• Lipase (metabolism)
• Liver (anatomy & histology)
• Peptide Hydrolases (metabolism)
• Phagosomes (drug effects, physiology)