p53-targeted lncRNA ST7-AS1 acts as a tumour suppressor by interacting with PTBP1 to suppress the Wnt/β-catenin signalling pathway in glioma.

Abstract

Glioma is the most prevalent intracranial tumour, with considerable morbidity. Long non-coding RNAs are important in the biological processes of various cancers. However, little is known about ST7 antisense RNA 1 (ST7-AS1) and its role in glioma progression. ST7-AS1 expression was reduced in glioma tissues and cells in comparison to normal brain tissues. p53 transcriptionally targeted the ST7-AS1 promoter in U251 glioma cells. The targeting significantly inhibited cell migration, invasion, and proliferation, and promoted apoptosis. ST7-AS1 directly bound to and downregulated polypyrimidine tract-binding protein 1 (PTBP1) at the post-transcriptional level. ST7-AS1 overexpression inhibited glioma progression by suppressing Wnt/β-catenin signalling by downregulating PTBP1 expression. Additionally, p53 expression negatively correlated with PTBP1 expression. Glioma progression is regulated by a positive feedback loop involving the p53/ST7-AS1/PTBP1 axis, which might be a promising therapeutic target for glioma treatment.

• Authors: Jie Sheng, Xin He, Wei Yu, Yingxi Chen, Yuxiang Long, Kejian Wang, Shujuan Zhu, Qian Liu
• Journal: Cancer letters (Cancer Lett) Vol. 503 Pg. 54-68 (04 10 2021) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 33476649 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Heterogeneous-Nuclear Ribonucleoproteins
• PTBP1 protein, human
• RNA, Long Noncoding
• TP53 protein, human
• Tumor Suppressor Protein p53
• Polypyrimidine Tract-Binding Protein

Topics

• Animals
• Apoptosis
• Brain Neoplasms (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Disease Progression
• Feedback, Physiological
• Gene Expression Regulation, Neoplastic
• Glioma (genetics, metabolism, pathology)
• Heterogeneous-Nuclear Ribonucleoproteins (genetics)
• Humans
• Male
• Mice
• Neoplasm Transplantation
• Polypyrimidine Tract-Binding Protein (genetics)
• Promoter Regions, Genetic
• RNA, Long Noncoding (genetics)
• Tumor Suppressor Protein p53 (metabolism)
• Wnt Signaling Pathway