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Establishment of a pigmented murine model abundant with characteristics of retinal vein occlusion.

Abstract
Retinal vein occlusion (RVO) is a vascular disease that represents characteristic retinal hemorrhage and dilated retinal veins. Despite its clinical importance, its pathogenesis remains largely unknown because of limited opportunities to acquire human retinal samples. Therefore, an animal model that reproduces the clinical features of RVO patients is required for further investigation. In this study, we established a pigmented murine RVO model that reproduced characteristic fundus appearances similar to human RVO findings. Retinal edema in this model was observed in both optical coherence tomography and histological analysis, which is a clinically important outcome. With quantitative real-time PCR analysis on retinal samples, we revealed that the mRNA level of vascular endothelial growth factor (VEGF) increased in the retina induced RVO. Moreover, this retinal edema was reduced by intravitreal injection of anti-VEGF antibody. These results were consistent with human clinical knowledge and suggested that this model could be a useful tool for research into new therapeutic approaches.
AuthorsSugao Miyagi, Anri Nishinaka, Takumi Yamamoto, Wataru Otsu, Shinsuke Nakamura, Masamitsu Shimazawa, Takashi Kitaoka, Hideaki Hara
JournalExperimental eye research (Exp Eye Res) Vol. 204 Pg. 108441 (03 2021) ISSN: 1096-0007 [Electronic] England
PMID33453278 (Publication Type: Journal Article)
CopyrightCopyright © 2021 Elsevier Ltd. All rights reserved.
Chemical References
  • Antibodies, Anti-Idiotypic
  • Immunoglobulin G
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, mouse
Topics
  • Animals
  • Antibodies, Anti-Idiotypic (therapeutic use)
  • Disease Models, Animal
  • Fluorescein Angiography
  • Humans
  • Immunoglobulin G (immunology)
  • Macular Edema (diagnosis, drug therapy, pathology)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger (genetics)
  • Real-Time Polymerase Chain Reaction
  • Retinal Vein Occlusion (diagnostic imaging, drug therapy, pathology)
  • Tomography, Optical Coherence
  • Treatment Outcome
  • Vascular Endothelial Growth Factor A (genetics, immunology)

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