Knockdown of CK2α reduces P-cresol-induced fibrosis in human renal proximal tubule epithelial cells via the downregulation of profilin-1.

Abstract

Renal fibrosis is one of the main causes of chronic kidney disease. Many studies have focused on fibroblasts and myofibroblasts involved in renal fibrogenesis. Recently, several studies have reported that renal proximal tubule epithelial cells are possible initiators of renal fibrosis. However, the mechanism through which cells induce renal fibrosis is poorly understood. In this study, we found that CK2α induces fibrosis in renal proximal tubule epithelial cells (TH1) by regulating the expression of profilin-1 (Pfn1). CKD mouse model and TH1 cells treated with P-cresol also showed an increased level of Pfn1. The knockdown of CK2α suppressed fibrosis in TH1 cells via the downregulation of Pfn1. In particular, CK2α knockdown inhibited the expression of stress fibers and fibrosis-related proteins in P-cresol-treated TH1 cells. Furthermore, the knockdown of CK2α inhibited mitochondrial dysfunction and restored cellular senescence and cell cycle in P-cresol-treated TH1 cells. These results indicate that CK2α induces renal fibrosis through Pfn1, which makes CK2α a key target molecule in the treatment of fibrosis related to chronic kidney disease.

• Authors: Yeo Min Yoon, Gyeongyun Go, Chul Won Yun, Ji Ho Lim, Sang Hun Lee
• Journal: International journal of medical sciences (Int J Med Sci) Vol. 17 Issue 17 Pg. 2850-2860 ( 2020) ISSN: 1449-1907 [Electronic] Australia
• PMID: 33162813 (Publication Type: Journal Article)
• Copyright: © The author(s).

Chemical References

• Cresols
• PFN1 protein, human
• Pfn1 protein, mouse
• Profilins
• 4-cresol
• CSNK2A1 protein, human
• Casein Kinase II
• Adenine

Topics

• Adenine (administration & dosage, toxicity)
• Animals
• Casein Kinase II (genetics, metabolism)
• Cell Line
• Cellular Senescence
• Cresols (toxicity)
• Disease Models, Animal
• Epithelial Cells
• Fibrosis
• Gene Knockdown Techniques
• Humans
• Kidney Tubules, Proximal (drug effects, pathology)
• Male
• Profilins (blood, genetics, metabolism)
• Renal Insufficiency, Chronic (blood, chemically induced, pathology)