Abstract | INTRODUCTION: METHODS: RESULTS: [131I] MIBG uptake was significantly higher in OAT1, OAT2, OCT1, and OCT2 than in mock cells. Uptake via OCT1 and OCT2 was little inhibited by MK-571 and probenecid. [131I] MIBG uptake into vesicles that highly expressed MRP1 or MRP4 was significantly higher in ATP than in AMP, and these inhibitors restored uptake to levels similar to that in AMP. Examining the time activity curves for [131I] MIBG in SK-N-SH cells, higher expressions of MDR1, MRP1, MRP4, and MK-571, or probenecid loading produced significantly higher uptake than in control at most incubation times. The ratios of tumors to blood or muscle in SK-N-SH-bearing mice were significantly increased by probenecid loading in comparison with normal mice. CONCLUSIONS: IMPLICATIONS FOR PATIENT CARE: Loading with probenecid, OAT, and MRP inhibitors improves [131I] MIBG accumulation.
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Authors | Masato Kobayashi, Asuka Mizutani, Kodai Nishi, Yuka Muranaka, Ryuichi Nishii, Naoto Shikano, Takeo Nakanishi, Ikumi Tamai, Eugenie S Kleinerman, Keiichi Kawai |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
2020 Nov - Dec
Vol. 90-91
Pg. 49-54
ISSN: 1872-9614 [Electronic] United States |
PMID | 33032192
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- Multidrug Resistance-Associated Proteins
- 3-Iodobenzylguanidine
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Topics |
- 3-Iodobenzylguanidine
(metabolism)
- Animals
- Biological Transport
- Cell Line, Tumor
- HEK293 Cells
- Humans
- Mice
- Multidrug Resistance-Associated Proteins
(metabolism)
- Neuroblastoma
(pathology)
- Tissue Distribution
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