Co-delivery of doxorubicin and aptamer against Forkhead box M1 using chitosan-gold nanoparticles coated with nucleolin aptamer for synergistic treatment of cancer cells.

Abstract

Herein, a nanotherapeutic delivery method was presented for co-delivery of doxorubicin (DOX) and aptamer against Forkhead box M1 (FOXM1 Apt) to cancer cells. Firstly, the vehicle composed of chitosan (CS)-Gold nanoparticles (AuNPs) conjugate was prepared. Nucleolin aptamer (AS1411) and FOXM1 Apt were loaded onto the CS-AuNPs and formed Aptamers (Apts)-CS-AuNPs. Subsequently, DOX was added to the Apts-CS-AuNPs to obtain the DOX-Apts-CS-AuNPs complex for synergistic treatment of tumor. The data of flow cytometry analysis and fluorescence imaging displayed that the complex was effectively internalized into target cells (A549 and 4T1 cells, nucleolin+) but not into CHO cells as nontarget cells. The results of the MTT assay showed that the complex significantly increased cell mortality in 4T1 and A549 cells compared to CHO cells treated with the complex. The in vivo studies demonstrated that the DOX-Apts-CS-AuNPs complex exhibited more tumor inhibitory effect and less distribution in other organs compared to free DOX.

• Authors: Zahra Khademi, Parirokh Lavaee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 248 Pg. 116735 (Nov 15 2020) ISSN: 1879-1344 [Electronic] England
• PMID: 32919550 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Antibiotics, Antineoplastic
• Aptamers, Nucleotide
• FOXM1 protein, human
• Forkhead Box Protein M1
• Phosphoproteins
• RNA-Binding Proteins
• Gold
• Doxorubicin
• Chitosan

Topics

• A549 Cells
• Animals
• Antibiotics, Antineoplastic (administration & dosage, pharmacokinetics)
• Aptamers, Nucleotide (administration & dosage, genetics, pharmacokinetics)
• CHO Cells
• Cell Line, Tumor
• Chitosan (chemistry)
• Cricetinae
• Cricetulus
• Doxorubicin (administration & dosage, pharmacokinetics)
• Drug Delivery Systems (methods)
• Drug Liberation
• Forkhead Box Protein M1 (genetics)
• Gold (chemistry)
• Humans
• Metal Nanoparticles (chemistry, ultrastructure)
• Mice, Inbred BALB C
• Microscopy, Atomic Force
• Microscopy, Electron, Scanning
• Neoplasms, Experimental (drug therapy, metabolism, pathology)
• Phosphoproteins (chemistry, genetics)
• RNA-Binding Proteins (chemistry, genetics)
• Nucleolin