Abstract | BACKGROUND: MATERIAL AND METHODS: For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical expression with different variables, considering p<0.05. Kaplan-Meier curves were produced for local recurrence, regional metastasis and treatment. RESULTS: A mRNA overexpression of ALDH1 in primary tumors was associated with regional metastasis and a high ALDH1 immunostaining was related to metastasis and a worse patient outcome. Additionally, a favorable outcome was associated with the transition phase and an unfavorable outcome was associated with EMT event. An overall 26.9 months was observed with longer survival associated with surgery and radiotherapy. CONCLUSIONS: However, due to the intense variability inherent to the indicator proteins in the EMT process, the complete profile markers related to this biological process should be continuous investigated.
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Authors | V Vieira, L-H Campos, L-H Jesus, C Klabunde, T-D Gamba, I-L Flores, M-G Oliveira, P-V Rados |
Journal | Medicina oral, patologia oral y cirugia bucal
(Med Oral Patol Oral Cir Bucal)
Vol. 25
Issue 6
Pg. e752-e761
(Nov 01 2020)
ISSN: 1698-6946 [Electronic] Spain |
PMID | 32701933
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Aldehyde Dehydrogenase 1 Family
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Topics |
- Aldehyde Dehydrogenase 1 Family
- Biomarkers, Tumor
- Epithelial-Mesenchymal Transition
- Head and Neck Neoplasms
- Humans
- Neoplasm Recurrence, Local
- Squamous Cell Carcinoma of Head and Neck
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