Identification of a HSP14-3-3 in Setaria cervi and its cross-reactivity with W bancrofti-infected human sera.

Abstract

AIM:

Identification of a 29 kDa heat stress protein in filarial parasite Setaria cervi and evaluation of its diagnostic potential against lymphatic filariasis.

METHODS AND RESULTS:

The Heat shock proteins (HSPs) were induced in filarial parasite S cervi by incubated at 42°C for 2 hours. The 10% SDS-PAGE of cytosolic extract showed several over-expressed bands. The MALDI-LC/MS analysis of 29 kDa band showed 100% similarity with Bm14-3-3 like protein 2. Multiple sequence alignment of Bm14-3-3 like protein 2 sequence with W bancrofti, Caenorhabditis elegans; Loa loa and Homo sapiens showed 100%, 86%, 83% and 78%, sequence similarity respectively. The antigenic efficacy of Sc14-3-3 protein was evaluated with different filarial sera using ELISA which showed cross-reactivity in order to Endemic Normal (EN) < Microfilaraemic (MF) < Chronic(CH) with IgG1 and EN < CH < MF in IgG4 ELISA. IgG1- and IgG4-specific immunoblotting with CH and MF sera further explicated its specific antigenic cross-reactivity.

CONCLUSION:

A 29 kDa heat shock protein of S cervi was identified as 14-3-3 protein having 100% homology to human filarial parasite B malayi. It showed strong reactivity with IgG1 and IgG4 subclass antibodies of W bancrofti-infected human sera suggesting that 14-3-3 protein could be used as a vaccine/ diagnostic marker.

• Authors: Faiyaz Ahmad, Ranjeet Kumar, Sarika Gupta, Sushma Rathaur
• Journal: Parasite immunology (Parasite Immunol) Vol. 42 Issue 11 Pg. e12777 (11 2020) ISSN: 1365-3024 [Electronic] England
• PMID: 32681576 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 John Wiley & Sons Ltd.

Chemical References

• Antibodies, Helminth
• Biomarkers
• Heat-Shock Proteins
• Helminth Proteins
• Immunoglobulin G

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Helminth (immunology)
• Biomarkers (analysis)
• Cross Reactions
• Elephantiasis, Filarial (diagnosis, immunology, parasitology)
• Female
• Heat-Shock Proteins (genetics, immunology, metabolism)
• Helminth Proteins (genetics, immunology, metabolism)
• Humans
• Immunoglobulin G (immunology)
• Sequence Alignment
• Setaria Nematode (genetics, immunology)
• Wuchereria bancrofti (immunology)