Ara h 2 is a relevant peanut
allergen linked to severe
allergic reactions. The interaction of
Ara h 2 with components of the sensitization phase of
food allergy (e.g., dendritic cells) has not been investigated, and could be key to understanding the allergenic potential of this
allergen. In this study, we aimed to analyze such interactions and the possible mechanism involved.
Ara h 2 was purified from two forms of peanut, raw and roasted, and labeled with a
fluorescent dye. Human monocyte-derived dendritic cells (MDDCs) were obtained, and experiments of
Ara h 2 internalization by MDDCs were carried out. The role of the
mannose receptor in the internalization of
Ara h 2 from raw and roasted peanuts was also investigated. Results showed that
Ara h 2 internalization by MDDCs was both time and dose dependent.
Mannose receptors in MDDCs had a greater implication in the internalization of
Ara h 2 from roasted peanuts. However, this receptor was also important in the internalization of
Ara h 2 from raw peanuts, as opposed to other
allergens such as raw
Ara h 3.