Phosphatidylinositol 4-kinase III beta regulates cell shape, migration, and focal adhesion number.

Abstract

Cell shape is regulated by cell adhesion and cytoskeletal and membrane dynamics. Cell shape, adhesion, and motility have a complex relationship and understanding them is important in understanding developmental patterning and embryogenesis. Here we show that the lipid kinase phosphatidylinositol 4-kinase III beta (PI4KIIIβ) regulates cell shape, migration, and focal adhesion (FA) number. PI4KIIIβ generates phosphatidylinositol 4-phosphate (PI4P) from phosphatidylinositol and is highly expressed in a subset of human breast cancers. PI4KIIIβ and the PI4P it generates regulate a variety of cellular functions, ranging from control of Golgi structure, fly fertility, and Akt signaling. Here, we show that loss of PI4KIIIβ expression decreases cell migration and alters cell shape in NIH3T3 fibroblasts. The changes are accompanied by an increase in the number of FA in cells lacking PI4KIIIβ. Furthermore, we find that PI4P-containing vesicles move to the migratory leading edge during migration and that some of these vesicles tether to and fuse with FA. Fusion is associated with FA disassembly. This suggests a novel regulatory role for PI4KIIIβ and PI4P in cell adhesion and cell shape maintenance.

• Authors: Patricia Bilodeau, Daniel Jacobsen, Denise Law-Vinh, Jonathan M Lee
• Journal: Molecular biology of the cell (Mol Biol Cell) Vol. 31 Issue 17 Pg. 1904-1916 (08 01 2020) ISSN: 1939-4586 [Electronic] United States
• PMID: 32583740 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Inositol Phosphates
• Phosphatidylinositol Phosphates
• phosphatidylinositol 4-phosphate
• Phosphotransferases (Alcohol Group Acceptor)
• 1-Phosphatidylinositol 4-Kinase
• phosphatidylinositol 4-kinase IIIbeta, human

Topics

• 1-Phosphatidylinositol 4-Kinase (metabolism)
• Animals
• Cell Adhesion (physiology)
• Cell Line, Tumor
• Cell Membrane (metabolism)
• Cell Movement (physiology)
• Cell Shape (physiology)
• Focal Adhesions (physiology)
• Golgi Apparatus (metabolism)
• Humans
• Inositol Phosphates (metabolism)
• Mice
• NIH 3T3 Cells
• Phosphatidylinositol Phosphates (metabolism)
• Phosphotransferases (Alcohol Group Acceptor) (metabolism)
• Signal Transduction (physiology)