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Immunotherapy with IgY Antibodies toward Outer Membrane Protein F Protects Burned Mice against Pseudomonas aeruginosa Infection.

Abstract
Burn patients with multidrug-resistant Pseudomonas aeruginosa infections commonly suffer from high morbidity and mortality, which present a major challenge to healthcare systems throughout the world. Outer membrane protein F (OprF), as a main outer membrane porin, is required for full virulence expression of P. aeruginosa. The aim of this study was to evaluate the protective efficacy of egg yolk-specific antibody (IgY) raised against recombinant OprF (r-OprF) protein in a murine burn model of infection. The hens were immunized with r-OprF, and anti-r-OprF IgY was purified using salt precipitation. Groups of mice were injected with different regimens of anti-OprF IgY or control IgY (C-IgY). Infections were caused by subcutaneous injection of P. aeruginosa strain PAO1 at the burn site. Mice were monitored for mortality for 5 days. The functional activity of anti-OprF IgY was determined by in vitro invasion assays. Immunotherapy with anti-OprF IgY resulted in a significant improvement in the survival of mice infected by P. aeruginosa from 25% to 87.5% compared with the C-IgY and PBS. The anti-OprF IgY decreased the invasion of P. aeruginosa PAO1 into the A549. Passive immunization with anti-OprF IgY led to an efficacious protection against P. aeruginosa burn infection in the burn model.
AuthorsFatemeh Norouzi, Bahador Behrouz, Mahya Ranjbar, Seyed Latif Mousavi Gargari
JournalJournal of immunology research (J Immunol Res) Vol. 2020 Pg. 7840631 ( 2020) ISSN: 2314-7156 [Electronic] Egypt
PMID32566689 (Publication Type: Journal Article)
CopyrightCopyright © 2020 Fatemeh Norouzi et al.
Chemical References
  • IgY
  • Immunoglobulins
  • OmpF protein
  • Porins
  • Recombinant Proteins
Topics
  • Animals
  • Antibody Specificity (immunology)
  • Burns (complications)
  • Disease Models, Animal
  • Immunoglobulins (isolation & purification, pharmacology, therapeutic use)
  • Immunotherapy
  • Male
  • Mice
  • Porins (immunology)
  • Prognosis
  • Pseudomonas Infections (etiology, immunology, mortality, therapy)
  • Pseudomonas aeruginosa (immunology)
  • Recombinant Proteins (isolation & purification, pharmacology)
  • Treatment Outcome

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