IL-10 and Its Related Superfamily Members IL-19 and IL-24 Provide Parallel/Redundant Immune-Modulation in Loa loa Infection.

Abstract

BACKGROUND:

Interleukin-10 (IL-10) has been implicated as the major cytokine responsible for the modulation of parasite-specific responses in filarial infections; however, the role of other IL-10 superfamily members in filarial infection is less well studied.

METHODS:

Peripheral blood mononuclear cells from loiasis patients were stimulated with or without filarial antigen. Cytokine production was quantified using a Luminex platform and T-cell expression patterns were assessed by flow cytometry.

RESULTS:

All patients produced significant levels of IL-10, IL-13, IL-5, IL-4, and IL-9 in response to filarial antigen, indicating a common infection-driven response. When comparing microfilaria (mf)-positive and mf-negative patients, there were no significant differences in spontaneous cytokine nor in parasite-driven IL-10, IL-22, or IL-28a production. In marked contrast, mf-positive individuals had significantly increased filarial antigen-driven IL-24 and IL-19 compared to mf-negative subjects. mf-positive patients also demonstrated significantly higher frequencies of T cells producing IL-19 in comparison to mf-negative patients. T-cell expression of IL-19 and IL-24 was positively regulated by IL-10 and IL-1β. IL-24 production was also regulated by IL-37.

CONCLUSION:

These data provide an important link between IL-10 and its related family members IL-19 and IL-24 in the modulation of the immune response in human filarial infections.

CLINICAL TRIALS REGISTRATION:

NCT00001230.

• Authors: Alessandra Ricciardi, Thomas B Nutman
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 223 Issue 2 Pg. 297-305 (02 03 2021) ISSN: 1537-6613 [Electronic] United States
• PMID: 32561912 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)
• Copyright: Published by Oxford University Press for the Infectious Diseases Society of America 2020.

Chemical References

• Cytokines
• IL10 protein, human
• IL19 protein, human
• Interleukins
• interleukin-24
• Interleukin-10

Topics

• Adolescent
• Adult
• Animals
• Child
• Cytokines (biosynthesis)
• Disease Susceptibility
• Female
• Host-Parasite Interactions
• Humans
• Immunomodulation
• Interleukin-10 (metabolism)
• Interleukins (metabolism)
• Loa (immunology)
• Loiasis (etiology, metabolism)
• Male
• Middle Aged
• T-Lymphocyte Subsets (immunology, metabolism)
• Th2 Cells (immunology, metabolism)
• Young Adult