Antigenic membrane proteins of virulent variant of Entamoeba histolytica HM-1:IMSS.

Abstract

Amoebiasis, caused by Entamoeba histolytica, is one of the leading parasitic infections in the world. This study was aimed at profiling antigenic membrane proteins of a virulent variant of E. histolytica HM-1:IMSS. The membrane proteins were extracted using ProteoExtract® kit (Merck, Darmstadt, Germany) or conventional method, separated using OFFGEL 3100 fractionator (Agilent Technologies, Santa Clara, California), followed by SDS-PAGE and Western blot analysis. Selected antigenic membrane proteins were identified using LC-ESI-MS/MS. Subsequently, the proteins were classified according to their biological processes and predictions were made on membrane and membrane-associated proteins. When the proteins were probed with pooled sera from amoebic liver abscess (ALA) patients, 10 and 15 antigenic proteins with molecular weights 25 to 200 kDa were identified using the ProteoExtract® kit and conventional method, respectively. LC-ESI-MS/MS identified 13 antigenic proteins, and both extraction methods predicted six of them as membrane and membrane-associated proteins. The topmost biological processes which comprised of six proteins were involved in cellular processes.. These antigenic membrane proteins merit further investigations as potential candidates for vaccine studies.

• Authors: Gaayathri Kumarasamy, Asmahani Azira Abdus Sani, Alfonso Olivos-García, Rahmah Noordin, Nurulhasanah Othman
• Journal: Pathogens and global health (Pathog Glob Health) Vol. 114 Issue 6 Pg. 333-342 (09 2020) ISSN: 2047-7732 [Electronic] England
• PMID: 32536281 (Publication Type: Journal Article)

Chemical References

• Membrane Proteins
• Protozoan Proteins

Topics

• Blotting, Western
• Dysentery, Amebic
• Entamoeba histolytica
• Humans
• Liver Abscess, Amebic
• Membrane Proteins (immunology)
• Protozoan Proteins (immunology)
• Tandem Mass Spectrometry