OSW-1 is a natural compound extracted from the bulbs of Ornithogalum saundersiae in 1992. It has been shown strong antitumor activities in various
cancer cells. However, the effects of
OSW-1 on
tumor growth and
metastasis in
breast cancer are still poorly understood. In our research, we showed that
OSW-1 had a strong anticancer effect on
breast cancer cells, but lower toxicity to normal cells. Accordingly, it also revealed significant inhibition of
tumor growth by
OSW-1 in xenograft model. In addition, we performed
Annexin V/PI-labeled flow cytometric assay and TUNEL assay and showed that
OSW-1 inhibited
tumor growth by inducing apoptosis. Furthermore, we carried out transwell assays and found that
OSW-1 significantly repressed the migratory and invasive capabilities of
triple-negative breast cancer (TNBC) cells via mediating epithelial-mesenchymal transition. Besides,
OSW-1 also could inhibit
metastasis in an orthotopic model and resulted in a longer survival compared with control group. Finally, we performed
RNA-sequencing and cellular functions to investigate the molecular mechanism of how
OSW-1 inhibits TNBC, and identified NFATc2 may as a pivotal factor for OSW-1-mediated effects on cell death,
tumor growth, invasion, and migration.