Pharmacokinetic and bioavailability studies of α-viniferin after intravenous and oral administration to rats.

Abstract

Alpha-viniferin is a trimer of resveratrol and has various pharmacological activities including anti-Alzheimer's disease, anti-tuberculosis, anti-tumor, anti-inflammatory and anti-diabetic. To investigate the pharmacokinetic characteristics and absolute bioavailability of α-viniferin in rats, using naringenin as an internal standard (IS), a rapid HPLC-MS/MS method of 5 min complete run time was developed. The chromatographic separation of α-viniferin and naringenin were accomplished with Waters XBridge™ C18 column (2.1 mm × 100 mm, 3.5 μm) and the mobile phase were acetonitrile and 0.1 % formic acid at a flow rate of 0.3 mL/min. Plasma samples were pretreated by ethyl acetate. The negative ion mode with electrospray ionization (ESI) source was used for detecting the sample. Oral bioavailability of α-viniferin was 4.2 %. This study will be beneficial in better understanding the pharmacological properties and the further development of α-viniferin.

• Authors: Yuqi Fan, Lulu Zhao, Xuhua Huang, Qi Jia, Wei Wang, Mengyuan Gao, Xiaohua Jia, Yanxu Chang, Huizi Ouyang, Jun He
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 188 Pg. 113376 (Sep 05 2020) ISSN: 1873-264X [Electronic] England
• PMID: 32502955 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Benzofurans
• alpha-viniferin

Topics

• Administration, Intravenous
• Administration, Oral
• Animals
• Benzofurans
• Biological Availability
• Chromatography, High Pressure Liquid
• Rats
• Reproducibility of Results
• Spectrometry, Mass, Electrospray Ionization
• Tandem Mass Spectrometry