N-Aryl-3-mercaptosuccinimides as Antivirulence Agents Targeting Pseudomonas aeruginosa Elastase and Clostridium Collagenases.

Abstract

In light of the global antimicrobial-resistance crisis, there is an urgent need for novel bacterial targets and antibiotics with novel modes of action. It has been shown that Pseudomonas aeruginosa elastase (LasB) and Clostridium histolyticum (Hathewaya histolytica) collagenase (ColH) play a significant role in the infection process and thereby represent promising antivirulence targets. Here, we report novel N-aryl-3-mercaptosuccinimide inhibitors that target both LasB and ColH, displaying potent activities in vitro and high selectivity for the bacterial over human metalloproteases. Additionally, the inhibitors demonstrate no signs of cytotoxicity against selected human cell lines and in a zebrafish embryo toxicity model. Furthermore, the most active ColH inhibitor shows a significant reduction of collagen degradation in an ex vivo pig-skin model.

• Authors: Jelena Konstantinović, Samir Yahiaoui, Alaa Alhayek, Jörg Haupenthal, Esther Schönauer, Anastasia Andreas, Andreas M Kany, Rolf Müller, Jesko Koehnke, Fabian K Berger, Markus Bischoff, Rolf W Hartmann, Hans Brandstetter, Anna K H Hirsch
• Journal: Journal of medicinal chemistry (J Med Chem) Vol. 63 Issue 15 Pg. 8359-8368 (08 13 2020) ISSN: 1520-4804 [Electronic] United States
• PMID: 32470298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Bacterial Proteins
• Matrix Metalloproteinase Inhibitors
• Succinimides
• Collagenases
• Metalloendopeptidases
• pseudolysin, Pseudomonas aeruginosa

Topics

• Animals
• Anti-Bacterial Agents (chemistry, pharmacology)
• Bacterial Proteins (antagonists & inhibitors, metabolism)
• Cell Line
• Clostridium Infections (drug therapy)
• Clostridium histolyticum (drug effects, enzymology)
• Collagenases (metabolism)
• Humans
• Matrix Metalloproteinase Inhibitors (chemistry, pharmacology)
• Metalloendopeptidases (antagonists & inhibitors, metabolism)
• Pseudomonas Infections (drug therapy)
• Pseudomonas aeruginosa (drug effects, enzymology)
• Succinimides (chemistry, pharmacology)
• Swine
• Zebrafish