Daucosterol from Crateva adansonii DC (Capparaceae) reduces 7,12-dimethylbenz(a)anthracene-induced mammary tumors in Wistar rats.

Abstract

This study aimed to evaluate the in vivo anticancer effects of daucosterol which was earlier reported to possess in vitro anticancer effects. Breast tumor was induced in 30 rats using the environmental carcinogen 7,12-dimethylbenz(a)anthracene (DMBA) while 6 control rats received olive oil (NOR). Animals with palpable tumors were randomized into five groups (n = 6) each as follows: negative control group treated with the vehicle (DMBA); positive control group treated with 5 mg/kg BW doxorubicin (DOXO + DMBA); three groups treated with daucosterol at doses of 2.5, 5, and 10 mg/kg BW (DAU + DMBA). Treatment lasted 28 days afterward, tumor (mass, volume, cancer antigen [CA] 15-3 level and histoarchitecture), hematological and toxicological parameters were examined. The tumor volume gradually increased in the DMBA group during the 28 days, with a tumor volume gain of ∼390 cm3 . Daucosterol at all doses reduced tumor volume (∼133.7 cm3 at 10 mg/kg) as well as protein, malondialdehyde (MDA), and CA 15-3 levels compared to DMBA rats. Tumor sections in daucosterol-treated rats showed a lower proliferation of mammary ducts with mild (5 and 10 mg/kg) to moderate (2.5 mg/kg) inflammatory responses. Moreover, it exhibited an antioxidant effect, evidenced by a significant and dose-dependent decreased in MDA levels, as well as an increase in catalase activity compared to the DMBA group. Daucosterol showed for the first time in vivo antitumor effects that corroborate its previous in vitro effects.

• Authors: Merline Ymele Nguedia, Alain Brice Tueche, Abel Joël Gbaweng Yaya, Vincent Yadji, Derek Tantoh Ndinteh, Dieudonné Njamen, Stéphane Zingue
• Journal: Environmental toxicology (Environ Toxicol) Vol. 35 Issue 10 Pg. 1125-1136 (Oct 2020) ISSN: 1522-7278 [Electronic] United States
• PMID: 32449848 (Publication Type: Journal Article)
• Copyright: © 2020 Wiley Periodicals LLC.

Chemical References

• Antineoplastic Agents, Phytogenic
• Antioxidants
• Carcinogens
• Sitosterols
• 9,10-Dimethyl-1,2-benzanthracene
• lyoniside

Topics

• 9,10-Dimethyl-1,2-benzanthracene (toxicity)
• Animals
• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology, therapeutic use)
• Antioxidants (metabolism)
• Capparaceae (chemistry)
• Carcinogens (toxicity)
• Dose-Response Relationship, Drug
• Female
• Mammary Neoplasms, Experimental (chemically induced, drug therapy, metabolism, pathology)
• Molecular Structure
• Plant Bark (chemistry)
• Rats
• Rats, Wistar
• Sitosterols (isolation & purification, pharmacology, therapeutic use)