MicroRNA-342 targets Cofilin 1 to suppress the growth, migration and invasion of human breast cancer cells.

Abstract

MicroRNA-342-3p (miR-342) has been shown to act as a tumor-suppressor in different cancer types. However, the role and therapeutic implications of miR-342 via modulation of Cofilin 1 (CFL1) has not been studied in any type of cancer. Given the importance of Cofilin signalling in breast, this study was undertaken to explore the therapeutic implications of miR-342 and its target CFL1 in breast cancer. Herein, we found that miR-342 was significantly (P < 0.05) downregulated in breast cancer tissues and cell lines. Functional assays revealed that overexpression of miR-342 caused a significant (P < 0.05) inhibition of the proliferation, colony formation, invasion and migration of the MDA-MB-436 and CAMA-1 breast cancer cells via induction of apoptosis. Bioinformatic approaches and the dual luciferase reporter assay confirmed the interaction between miR-342 and its target CFL1. Moreover, we found that CFL1 was aberrantly overexpressed in breast cancer tissues and cell lines. Overexpression of miR-342 caused remarkable depletion in the expression of CFL1 in MDA-MB-436 breast cancer cells. Silencing of CFL1 in CAMA-1 and MDA-MB-436 cells caused remarkable decrease in the proliferation, colony formation and migration of these cells, similar to that of miR-342 ovexpression. However, overexpression of CFL1 in MDA-MB-346 cells could avoid the tumor suppressive effects of miR-342. Our data provide novel information about the implications of miR-342 and its target CFL1 in breast cancer treatment.

• Authors: Cong Liu, Hua Xing, Xiao Luo, Yimin Wang
• Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 687 Pg. 108385 (07 15 2020) ISSN: 1096-0384 [Electronic] United States
• PMID: 32335050 (Publication Type: Journal Article)
• Copyright: Copyright © 2020. Published by Elsevier Inc.

Chemical References

• CFL1 protein, human
• Cofilin 1
• MIRN342 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Apoptosis (physiology)
• Breast Neoplasms (physiopathology)
• Cell Line, Tumor
• Cell Movement (physiology)
• Cell Proliferation (physiology)
• Cofilin 1 (metabolism)
• Down-Regulation (physiology)
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• MicroRNAs (metabolism)
• Middle Aged
• Up-Regulation (physiology)