Dietary Exposure to Oxidized Frying Oil from Fetus to Adulthood Suppresses Male Reproductive Development by Altering Testicular Cholesterol and Testosterone Homeostasis in Sprague Dawley Rats.

Abstract

BACKGROUND:

Dietary frying oil may have endocrine-disrupting effects, as a feminization effect was observed in cohorts of C57BL/6J male mice fetuses from dams consuming oxidized frying oil (OFO) during pregnancy.

OBJECTIVE:

The aim of present study was to test the hypothesis that OFO is an anti-androgen.

METHODS:

In experiment 1, male progeny of Sprague Dawley female rats fed fresh oil or an OFO diet (10 g fat/100 g, from fresh or 24-h-fried soybean oil; [control diet (C) and OFO groups, respectively] from midgestation through lactation were studied. Pups were weaned at 3 wk of age and then consumed their mothers' diet until 9 wk of age. In addition, a group of dams and pups that consumed a high-fat diet (HF; 10 g fried and 20 g fresh soybean oil/100 g) was included to counteract body-weight loss associated with OFO ingestion. Indices of male reproductive development and testosterone homeostasis were measured. In experiment 2, male rats were allocated to C and OFO groups (treated as above) and indices of male fertility compared at 9-10 wk of age.

RESULTS:

In experiment 1, final body weights of the HF group were lower (17%) than the C group but higher (14%) than the OFO group (P < 0.0001 for each). In addition to abnormalities in seminiferous tubules, HF and OFO groups did not differ from one another, but, compared with the C group, had delayed preputial separation (4.9 d) and reductions in serum testosterone concentrations (17-74%), anogenital distance (8-20%), weights of androgen-dependent tissues (8-30%), testicular testosterone and cholesterol concentrations (30-40%), and mRNA levels of genes involved in steroidogenesis and cholesterol homeostasis (30-70%). In experiment 2, OFO-exposed males had 20% lower sperm motility (P < 0.05); however, when mated to normal females, pregnancy rates and litter sizes did not differ between OFO and C groups.

CONCLUSIONS:

The anti-androgenic effect of OFO in Sprague Dawley rats was attributed to decreased testicular concentrations of cholesterol (testosterone precursor) and not body-weight loss.

• Authors: Hai-Ping Wu, Yu-Shun Lin, Chi-Fen Chang, Shui-Yuan Lu, Pei-Min Chao
• Journal: The Journal of nutrition (J Nutr) Vol. 150 Issue 7 Pg. 1713-1721 (07 01 2020) ISSN: 1541-6100 [Electronic] United States
• PMID: 32286625 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © The Author(s) on behalf of the American Society for Nutrition 2020.

Chemical References

• Dietary Fats
• Testosterone
• Soybean Oil
• Cholesterol

Topics

• Animal Nutritional Physiological Phenomena
• Animals
• Cholesterol (metabolism)
• Cooking
• Dietary Fats (administration & dosage, toxicity)
• Female
• Homeostasis (drug effects)
• Male
• Oxidation-Reduction
• Pregnancy
• Prenatal Exposure Delayed Effects
• Prenatal Nutritional Physiological Phenomena
• Rats
• Rats, Sprague-Dawley
• Soybean Oil (toxicity)
• Testis (drug effects, metabolism)
• Testosterone (metabolism)