Growing evidence has indicated that
circular RNAs (
circRNAs) play crucial roles in multiple biological processes. However, alterations in
circRNA profiles during
bladder cancer progression and the clinical significance thereof remain unclear. Therefore, high-throughput
RNA sequencing was conducted to identify
circRNA and
mRNA profiles in five pairs of
bladder cancer tissues and adjacent noncancerous tissues. A total of 87 differentially expressed
circRNAs and 2756 mRNAs were detected in above
bladder cancer samples compared with paired noncancerous samples. Functional enrichment analyses,
circRNA-
microRNA-
mRNA, and protein-protein interaction networks revealed that these dysregulated
circRNAs were potentially involved in
carcinogenesis and evolution of
bladder cancer. Subsequently, the differential expression of eight
circRNAs was detected by real-time qPCR. Hsa_circ_0003141 and hsa_circ_0008039 were significantly upregulated as well as hsa_circ_0026782, hsa_circ_0077837, hsa_circ_0004826, and hsa_circ_0001946 were significantly downregulated among validation of 70 matched
bladder cancer tissues (≥75%). Moreover, hsa_circ_0077837 and hsa_circ_0004826 were also verified as markedly downregulated in four
bladder cancer cells (100%). Naturally, hsa_circ_0077837 and hsa_circ_0004826 were also demonstrated using
RNase-R+ resistance experiments. In addition, Fisher's exact test, Kaplan-Meier plots, Cox regression analyses, and receiver operating characteristic curve was performed to assess their clinical value. Downregulation of hsa_circ_0077837 and hsa_circ_0004826 all was significantly correlated with worse clinicopathological features and poor prognosis of
bladder cancer patients. The area under the receiver operating characteristic curve of them was 0.775 (P < .0001) and 0.790 (P < .0001), respectively. Not surprisingly, in vitro functional experiments also demonstrated that the overexpression of hsa_circ_0077837 and hsa_circ_0004826 significantly weakened the proliferation, migration, and invasion of
bladder cancer cells. Overall, hsa_circ_0077837 and hsa_circ_0004826 might act as
tumor suppressors in the
bladder cancer progression and serve as a potential
biomarker for the diagnosis, prognosis, and
therapy of
bladder cancer.