Abstract | BACKGROUND:
Transplantation of skeletal myoblast sheets is a promising strategy for the treatment of heart failure, and its therapeutic effects have already been proven in both animal disease models and clinical trials. Myoblast sheets reportedly demonstrate their therapeutic effects by producing many paracrine factors. Although the quality of processed cells for transplantation can be evaluated by the positive ratio of CD56, a myoblast marker, it is unclear which cell populations from isolated cells produce paracrine factors that have an impact on therapeutic effects, and whether these therapeutic effects are closely correlated with CD56-positive cells isolated from the skeletal muscle is also unclear. Therefore, we hypothesized that CD56-negative cells as well as CD56-positive cells isolated from the skeletal muscle produce paracrine factors and have therapeutic effects in skeletal muscle-derived cell sheet therapy for heart failure. METHODS: RESULTS: Analysis of cell surface and intracellular markers revealed that CD56-negative NMCs expressed fibroblast markers and a higher level of mesenchymal cell markers, such as CD49b and CD140a, than myoblasts. Both NMCs and myoblasts expressed various cytokines in vitro with different expression patterns. In addition, NMCs induced tube formation (control vs. myoblasts vs. NMCs: 100 ± 11.2 vs. 142 ± 8.3 vs. 198 ± 7.4%) and stem cell mobilization (control vs. myoblasts vs. NMCs: 100 ± 6.8 vs. 210 ± 22.9 vs. 351 ± 36.0%) to a higher degree in vitro than did myoblasts. The effect of NMCs and myoblasts on the improvement of cardiac function and suppression of myocardial fibrosis in rat myocardial infarction model was comparable. CONCLUSION:
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Authors | Hiroko Iseoka, Shigeru Miyagawa, Atsuhiro Saito, Akima Harada, Yoshiki Sawa |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 11
Issue 1
Pg. 69
(02 18 2020)
ISSN: 1757-6512 [Electronic] England |
PMID | 32070429
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cell Differentiation
- Cells, Cultured
- Disease Models, Animal
- Echocardiography
(methods)
- Heart Failure
(pathology, therapy)
- Male
- Muscle, Skeletal
(cytology, metabolism)
- Myocardial Infarction
(pathology, therapy)
- Rats
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