Objective: To investigate the antagonistic effect of
diallyl sulfide (
DAS) against
peripheral nerve injury induced by
n-hexane in rats. Methods: A total of 68 adult male Wistar rats were selected, among which 50 were randomly selected and divided into blank control group,
DAS control group (100 mg/kg·bw) ,
n-hexane model group, low-dose
DAS intervention group (50 mg/kg·bw) , and high-dose
DAS intervention group (100 mg/kg·bw) . A rat model of
peripheral nerve injury was established by
n-hexane exposure, and the rats were treated with
DAS at different doses. The changes in
pyrrole adducts and behavior were observed, a metabolic analysis was performed for serum
pyrrole adducts, and the intervention effect was evaluated. The remaining 18 rats were randomly assigned to the
n-hexane model group, the low-dose
DAS intervention group, and the high-dose
DAS intervention group, with 6 rats in each group, as satellite groups used for the toxicokinetic analysis of serum
pyrrole adducts. Results: Compared with the blank control group, the
n-hexane model group and low-and high-dose
DAS intervention groups had a significant reduction in
body weight since week 2 (P<0.01) . Compared with the
n-hexane model group at the end of the experiment at week 7, the high-dose
DAS intervention group had a significantly higher
body weight (P<0.05) , while there was no significant difference in
body weight between the
n-hexane model group and the low-dose
DAS intervention group (P>0.05) . The
n-hexane model group developed gait abnormality at week 2 of
poisoning, while the low-and high-dose
DAS intervention groups developed gait abnormality at weeks 3 and 5 of
poisoning, respectively. At the end of the experiment, the
n-hexane model group and the low-and high-dose
DAS intervention groups had a significantly higher gait score than the blank control group (P<0.01) . At the end of the experiment, the
n-hexane model group and the low-dose
DAS intervention group had significantly shorter latency in rotarod test than the blank control group (P<0.01) , while there was no significant difference in latency between the
DAS control group and the high-dose
DAS intervention group (P>0.05) . Compared with the
n-hexane model group, the low-and high-dose
DAS intervention groups had a significant increase in latency in rotarod test (P<0.01) . Compared with blank control group, the
n-hexane model group and the low-dose
DAS intervention group had a significant increase in mean nerve conduction velocity (P<0.01) , while there was no significant difference between the blank control group and the
DAS control group or high-dose
DAS intervention group (P>0.05) , and compared with the
n-hexane model group, the low-and high-dose
DAS intervention groups had a significant increase in nerve conduction velocity (P<0.01) . Compared with the blank control group at the end of the experiment at week 7, the
n-hexane model group and the low-and high-dose
DAS intervention groups had significant increases in the concentration of
pyrrole adducts in serum, urine, and hair (P<0.01) , while there was no significant difference between the blank control group and the
DAS control group (P>0.05) , and the high-dose
DAS intervention group had a significantly lower concentration of
pyrrole adducts in serum, urine, and hair than the low-dose
DAS intervention group (P<0.05) . Serum
pyrrole adducts reached the peak level at 9-12 hours and then started to decrease. Compared with the
n-hexane model group, the high-and low-dose
DAS intervention groups had a significantly shorter half-life period of serum
pyrrole adducts (P<0.01) . Compared with the
n-hexane model group, the high-and low-dose
DAS intervention groups had a significant reduction in the area under the curve of serum
pyrrole adducts (P<0.05) . Conclusion:
DAS can antagonize
peripheral nerve injury induced by
n-hexane.