Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death.

Abstract

Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.

• Authors: Lenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek, Jitka Fucikova
• Journal: Methods in enzymology (Methods Enzymol) Vol. 632 Pg. 15-25 ( 2020) ISSN: 1557-7988 [Electronic] United States
• PMID: 32000894 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers, Tumor
• Calreticulin

Topics

• Animals
• Biomarkers, Tumor (analysis, immunology)
• Calreticulin (analysis, immunology)
• Flow Cytometry (methods)
• Humans
• Immunogenic Cell Death
• Immunohistochemistry (methods)
• Neoplasms (immunology, pathology)
• Tumor Microenvironment