Avertin affects murine colitis by regulating neutrophils and macrophages.

Abstract

Anesthetics are thought to be involved in immunomodulation. Avertin is one of the safest and most commonly used intravenous anesthetics in rodent experiments; it is also widely used in euthanasia of inflammatory bowel disease (IBD) models. This study aimed to define the role and mechanism of action of Avertin on murine colitis. We assessed the effects of a single Avertin injection on colitis using the disease activity index (DAI), pathology, enzyme-linked immunosorbent assay (ELISA), multiplex-ELISA, flow cytometry, and routine blood examination in wild-type (WT) and dextran sodium sulphate (DSS)-treated mice. Although Avertin caused acute cecitis in WT mice after 24 h and aggravated inflammation in the medium term, it alleviated inflammation in the late stage of DSS-induced colitis according to the DAI. Avertin upregulated MPO production and induced the accumulation of neutrophils and macrophages in intestinal mucosa of both WT and DSS-treated mice; the altered MPO might indicate a change in respiratory burst. However, it exhibited a more effective suppression of inflammatory factors secreted by macrophages as the colitis progressed. Avertin led to an increase in neutrophils and decrease in monocytes in both WT and DSS-treated mice blood. Our findings suggest that Avertin aggravates inflammation in the early and medium terms, but alleviates inflammation in the late stage of colitis by regulating neutrophils and macrophages.

• Authors: Dezhi Wang, Linxiao Chen, Yanxia Fu, Qian Kang, Xin Wang, Xianzong Ma, Xuhang Li, Jianqiu Sheng
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 80 Pg. 106153 (Mar 2020) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 31931369 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Anesthetics
• Anti-Inflammatory Agents
• tribromoethanol
• Ethanol
• Dextran Sulfate

Topics

• Anesthetics (therapeutic use)
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Cecum (drug effects, pathology)
• Colitis (chemically induced, drug therapy, immunology, pathology)
• Colon (drug effects, immunology)
• Dextran Sulfate
• Ethanol (analogs & derivatives, therapeutic use)
• Female
• Leukocyte Count
• Macrophages (drug effects, immunology)
• Male
• Mice, Inbred C57BL
• Neutrophils (drug effects, immunology)