Abstract |
Macroautophagy/autophagy functions as a part of the innate immune system in clearing intracellular pathogens. Although this process is well known, the mechanisms that control antibacterial autophagy are not clear. In this study we show that during intracellular Salmonella typhimurium infection, the activity of TFEB ( transcription factor EB), a master regulator of autophagy and lysosome biogenesis, is suppressed by maintaining it in a phosphorylated state on the lysosomes. Furthermore, we have identified a novel, antibacterial small molecule autophagy (xenophagy) modulator, acacetin. The xenophagy effect exerted by acacetin occurs in an MTOR (mechanistic target of rapamycin kinase)-independent, TFEB-dependent manner. Acacetin treatment results in persistently maintaining active TFEB in the nucleus and also in TFEB mediated induction of functional lysosomes that target Salmonella-containing vacuoles (SCVs). The enhanced proteolytic activity due to deployment of lysosomes results in clamping down Salmonella replication in SCVs. Acacetin is effective as a xenophagy compound in an in vivo mouse model of infection and reduces intracellular Salmonella burden. ABBREVIATIONS:
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Authors | Veena Ammanathan, Piyush Mishra, Aravinda K Chavalmane, Sasikumar Muthusamy, Vidya Jadhav, Chandrashekaran Siddamadappa, Ravi Manjithaya |
Journal | Autophagy
(Autophagy)
Vol. 16
Issue 9
Pg. 1584-1597
(09 2020)
ISSN: 1554-8635 [Electronic] United States |
PMID | 31744366
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
- Flavones
- TFEB protein, human
- acacetin
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Topics |
- Animals
- Autophagy
(drug effects)
- Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
(metabolism)
- Disease Models, Animal
- Flavones
(pharmacology)
- HeLa Cells
- Humans
- Intracellular Space
(microbiology)
- Lysosomes
(drug effects, metabolism, ultrastructure)
- Mice
- Phosphorylation
(drug effects)
- Proteolysis
(drug effects)
- RAW 264.7 Cells
- Saccharomyces cerevisiae
(drug effects, metabolism, ultrastructure)
- Salmonella typhimurium
(drug effects, growth & development)
- Vacuoles
(drug effects, metabolism, ultrastructure)
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