Co-delivery of GOLPH3 siRNA and gefitinib by cationic lipid-PLGA nanoparticles improves EGFR-targeted therapy for glioma.

Abstract

Glioblastoma is one of the most aggressive types of brain tumor. Epidermal growth factor receptors (EGFRs) are overexpressed in glioma, and EGFR amplifications and mutations lead to rapid proliferation and invasion. EGFR-targeted therapy might be an effective treatment for glioma. Gefitinib (Ge) is an EGFR tyrosine kinase inhibitor (TKI), and Golgi phosphoprotein 3 (GOLPH3) expression is associated with worse glioma prognosis. Downregulation of GOLPH3 could promote EGFR degradation. Here, an angiopep-2 (A2)-modified cationic lipid-poly (lactic-co-glycolic acid) (PLGA) nanoparticle (A2-N) was developed that can release Ge and GOLPH3 siRNA (siGOLPH3) upon entering glioma cells and therefore acts as a combinatorial anti-tumor therapy. The in vitro and in vivo studies proved that A2-N/Ge/siGOLPH3 successfully crossed the blood-brain barrier (BBB) and targeted glioma. Released siGOLPH3 effectively silenced GOLPH3 mRNA expression and further promoted EGFR and p-EGFR degradation. Released Ge also markedly inhibited EGFR signaling. This combined EGFR-targeted action achieved remarkable anti-glioma effects and could be a safe and effective treatment for glioma. KEY MESSAGES: Angiopep-2-modified cationic lipid polymer can penetrate the BBB. Gefitinib can inhibit EGFR signaling and block the autophosphorylation of critical tyrosine residues on EGFR. GOLPH3 siRNA can be transfected into glioma and downregulate GLOPH3 expression. A2-N/Ge/siGOLPH3 can inhibit glioma growth.

• Authors: Chengkun Ye, Bomin Pan, Haoyue Xu, Zongren Zhao, Jiawei Shen, Jun Lu, Rutong Yu, Hongmei Liu
• Journal: Journal of molecular medicine (Berlin, Germany) (J Mol Med (Berl)) Vol. 97 Issue 11 Pg. 1575-1588 (11 2019) ISSN: 1432-1440 [Electronic] Germany
• PMID: 31673738 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GOLPH3 protein, human
• GPP34 protein, mouse
• Membrane Proteins
• Phosphoproteins
• RNA, Small Interfering
• EGFR protein, human
• ErbB Receptors
• Gefitinib

Topics

• Animals
• Cell Proliferation (genetics, physiology)
• ErbB Receptors (genetics, metabolism)
• Gefitinib (therapeutic use)
• Gene Expression Regulation, Neoplastic (genetics, physiology)
• Glioma (drug therapy, metabolism)
• Humans
• Male
• Membrane Proteins (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Nanoparticles (chemistry)
• Phosphoproteins (genetics, metabolism)
• RNA, Small Interfering (genetics, metabolism)