Abstract | BACKGROUND: OBJECTIVE: This study evaluates the effects of Cer and Ytt Ox NPs and Nan Se on H2O2- induced oxidative stress in pancreatic beta cells with focus on mitochondrial dysfunction pathway. METHODS: CRI-D2 beta-cell line were pretreated with Cer Ox NPs (200 µM) + Ytt Ox NPs (0.5 µg/mL) for 3 days and/or Nan Se (0.01 µM) for 1 day. Then markers of oxidative stress, mitochondrial dysfunction, insulin and glucagon secretion were measured. RESULTS: We reported a decrease in H2O2-induced reactive oxygen species (ROS) level and glucagon secretion, and an increase in H2O2-reduced ATP/ ADP ratio, MMP, as well as UCP2 protein expression, and insulin secretion by pretreatment of CRI-D2 cells with Cer and Ytt Ox NPs and/or Nan Se. CONCLUSION: We found maximum protective effect with Cer and Ytt Ox NPs on CRI-D2 beta-cell line exposed by H2O2 for keeping beta cells alive until transplant whereas combination of Cer and Ytt Ox NPs and Nan Se had very little protective effect in this condition.
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Authors | Shima Tavoosi, Amir Hossein Baghsheikhi, Seyed Vahid Shetab-Boushehri, Mona Navaei-Nigjeh, Nazanin Namazi Sarvestani, Mohammad Yahya Karimi, Akram Ranjbar, Alireza Ebadollahi-Natanzi, Asieh Hosseini |
Journal | Pharmaceutical nanotechnology
(Pharm Nanotechnol)
Vol. 8
Issue 1
Pg. 63-75
( 2020)
ISSN: 2211-7393 [Electronic] United Arab Emirates |
PMID | 31577213
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright© Bentham Science Publishers; For any queries, please email at [email protected]. |
Chemical References |
- Antioxidants
- Insulin
- Ucp2 protein, rat
- Uncoupling Protein 2
- Cerium
- Yttrium
- ceric oxide
- Glucagon
- Hydrogen Peroxide
- Selenium
- yttria
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Topics |
- Animals
- Antioxidants
(chemistry, pharmacology)
- Apoptosis
(drug effects)
- Cell Line
- Cerium
(chemistry, pharmacology)
- Glucagon
(metabolism)
- Hydrogen Peroxide
(adverse effects)
- Insulin
(metabolism)
- Insulin-Secreting Cells
(cytology, drug effects, metabolism)
- Mitochondria
(drug effects, metabolism)
- Nanoparticles
- Oxidative Stress
(drug effects)
- Rats
- Selenium
(chemistry, pharmacology)
- Uncoupling Protein 2
(metabolism)
- Yttrium
(chemistry, pharmacology)
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