The role of endogenous thiamine produced via THIC in root nodule symbiosis in Lotus japonicus.
|
| Abstract |
Thiamine is a pivotal primary metabolite which is indispensable to all organisms. Although its biosynthetic pathway has been well documented, the mechanism by which thiamine influences the legume-rhizobium symbiosis remains uncertain. Here, we used overexpressing transgenic plants, mutants and grafting experiments to investigate the roles played by thiamine in Lotus japonicus nodulation. ljthic mutants displayed lethal phenotypes and the defect could be overcome by supplementation of thiamine or by overexpression of LjTHIC. Reciprocal grafting between L. japonicus wild-type Gifu B-129 and ljthic showed that the photosynthetic products of the aerial part made a major contribution to overcoming the nodulation defect in ljthic. Overexpression of LjTHIC in Lotus japonicus (OE-LjTHIC) decreased shoot growth and increased the activity of the enzymes 2-oxoglutarate dehydrogenase and pyruvate dehydrogenase. OE-LjTHIC plants exhibited an increase in the number of infection threads and also developed more nodules, which were of smaller size but unchanged nitrogenase activity compared to the wildtype. Taken together, our results suggest that endogenous thiamine produced via LjTHIC acts as an essential nutrient provided by the host plant for rhizobial infection and nodule growth in the Lotus japonicus - rhizobium interaction.
|
|---|---|
| Authors | Yehu Yin, Lu Tian, Xueliu Li, Mingchao Huang, Leru Liu, Pingzhi Wu, Meiru Li, Huawu Jiang, Guojiang Wu, Yaping Chen |
| Journal | Plant science : an international journal of experimental plant biology (Plant Sci) Vol. 283 Pg. 311-320 (Jun 2019) ISSN: 1873-2259 [Electronic] Ireland |
| PMID | 31128701 (Publication Type: Journal Article) |
| Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!