Transducin β-like 1 X-linked receptor 1 (TBLR1) affects RGNNV infection through negative regulation of interferon immune response in orange-spotted grouper, Epinephelus coioides.

Abstract

Transducin β-like 1 X-linked receptor 1 (TBLR1) was identified as an important component of nuclear receptor corepressor (N-CoR) complex, and functionally participated in regulation of transcriptional activation. However, the potential roles of TBLR1 in innate immune response still remain uncertain. In the present work, a novel TBLR1 from orange-spotted grouper, Epinephelus coioides (named as EcTBLR1) was cloned and its effect on fish virus infection was characterized. The full length open reading frame (ORF) of EcTBLR1 was 1548 bp and encoded a putative 515-aa polypeptide, which shared 99% and 95% identity with its homologue from large yellow croaker (Larimichthys crocea) and human (Homo sapiens), respectively. Quantitative PCR (qPCR) analysis revealed a ubiquitous expression of EcTBLR1 in different tissues with remarkable expression in brain, spleen and head-kidney. Subcellular location analysis showed that EcTBLR1 was mainly located in cytoplasm of grouper spleen cells, and partly translocated into nucleus after infection with red spotted grouper nervous necrosis virus (RGNNV). Moreover, RGNNV infection suppressed the protein synthesis of EcTBLR1 in grouper cells. Using RNA interference (RNAi) technology, we found that effective knock-down of EcTBLR1 significantly suppressed the transcription of RGNNV capsid protein (Cp) and RNA-dependent RNA polymerase (RdRp) genes, which implied the crucial role of EcTBLR1 in RGNNV infection. Consistently, overexpression of EcTBLR1 in vitro significantly inhibited IFN promoter activity, as well as the transcription of IFN-related downstream effectors, including interferon stimulated gene 15 (ISG15) and interferon regulatory factor 3 (IRF3). Together, our results for the first time demonstrated that fish TBLR1 might exert critical roles during fish RNA virus replication by negatively regulating interferon response.

• Authors: Songwei Ni, Jiaxin Liu, Xiaohong Huang, WenXiong Wang, Youhua Huang, Qiwei Qin
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 89 Pg. 76-82 (Jun 2019) ISSN: 1095-9947 [Electronic] England
• PMID: 30917925 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Fish Proteins
• Repressor Proteins
• Interferons

Topics

• Amino Acid Sequence
• Animals
• Bass (genetics, immunology)
• Fish Diseases (immunology)
• Fish Proteins (chemistry, genetics, immunology)
• Gene Expression Profiling (veterinary)
• Gene Expression Regulation (immunology)
• Immunity, Innate (genetics)
• Interferons (immunology)
• Nodaviridae (physiology)
• Phylogeny
• RNA Virus Infections (immunology, veterinary)
• Repressor Proteins (chemistry, genetics, immunology)
• Sequence Alignment (veterinary)