Allergenicity suppression of tropomyosin from Exopalaemon modestus by glycation with saccharides of different molecular sizes.

Abstract

The allergenicity suppression of tropomyosin (TM) from Exopalaemon modestus by glycation with saccharides of different molecular sizes (glucose, maltose, maltotriose, maltopentaose and maltoheptaose) was investigated using immunoblotting, human colon epithelial cell line (Caco-2) and human basophil cell line (KU812). Glycation of TM by glucose, maltotriose, maltopentaose and maltoheptaose significantly destructed and masked TM epitopes to obtain lower allergenicity, while glycation of TM by maltose had insignificant suppression on TM allergenicity. In addition, the glycated TM by glucose, maltotriose, maltopentaose and maltoheptaose inhibited the proliferation and IL-8 secretion of Caco-2, and the CD63 and CD203c expression, MAPK signaling of KU812 basophils, while the glycated TM by maltose had insignificant suppression on the allergy reactivities of Caco-2 cells and KU812 basophils. Glycation of TM by saccharides with larger molecular sizes (such as maltoheptaose) could provide new insight into the desensitization of shrimp-induced food allergy.

• Authors: Ziye Zhang, Hang Xiao, Peng Zhou
• Journal: Food chemistry (Food Chem) Vol. 288 Pg. 268-275 (Aug 01 2019) ISSN: 1873-7072 [Electronic] England
• PMID: 30902292 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Allergens
• Interleukin-8
• Oligosaccharides
• Trisaccharides
• Tropomyosin
• maltopentaose
• Immunoglobulin E
• maltotriose
• Maltose

Topics

• Adolescent
• Allergens (immunology)
• Animals
• Caco-2 Cells
• Cell Line
• Cell Proliferation (drug effects)
• Child, Preschool
• Female
• Glycosylation
• Humans
• Immunoglobulin E (blood)
• Interleukin-8 (metabolism)
• Male
• Maltose (metabolism)
• Middle Aged
• Oligosaccharides (metabolism)
• Palaemonidae (metabolism)
• Trisaccharides (metabolism)
• Tropomyosin (immunology, metabolism, pharmacology)