Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris.

Abstract

Pemphigus vulgaris (PV) is a potentially lethal mucocutaneous blistering disease characterized by IgG autoantibodies (AuAbs) binding to epidermal keratinocytes and inducing this devastating disease. Here, we observed that non-desmoglein (Dsg) AuAbs in the sera of patients with Dsg1/3 AuAb-negative acute PV are pathogenic, because IgGs from these individuals induced skin blistering in neonatal mice caused by suprabasal acantholysis. Serum levels of AuAbs to desmocollin 3 (Dsc3), M3 muscarinic acetylcholine receptor (M3AR), and secretory pathway Ca2+/Mn2+-ATPase isoform 1 (SPCA1) correlated with the disease stage of PV. Moreover, AuAb absorption on recombinant Dsc3, M3AR, or SPCA1 both prevented skin blistering in the passive transfer of AuAbs model of PV in BALB/c mice and significantly decreased the extent of acantholysis in a neonatal mouse skin explant model. Although acantholytic activities of each of these immunoaffinity-purified AuAbs could not induce a PV-like phenotype, their mixture produced a synergistic effect manifested by a positive Nikolskiy sign in the skin of neonatal mice. The downstream signaling of all pathogenic non-Dsg AuAbs involved p38 mitogen-activated protein kinase (MAPK)-mediated phosphorylation and elevation of cytochrome c release and caspase 9 activity. Anti-Dsc3 and anti-SPCA1 AuAbs also activated SRC proto-oncogene, nonreceptor tyrosine kinase (SRC). Of note, although a constellation of non-Dsg AuAbs apparently disrupted epidermal integrity, elimination of a single pathogenic AuAb could prevent keratinocyte detachment and blistering. Therefore, anti-Dsg1/3 AuAb-free PV can be a model for elucidating the roles of non-Dsg antigen-specific AuAbs in the physiological regulation of keratinocyte cell-cell adhesion and blister development.

• Authors: Alex Chernyavsky, Kyle T Amber, Arianna F Agnoletti, Candice Wang, Sergei A Grando
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 294 Issue 12 Pg. 4520-4528 (03 22 2019) ISSN: 1083-351X [Electronic] United States
• PMID: 30692201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 Chernyavsky et al.

Chemical References

• Autoantibodies
• Desmoglein 1
• Desmoglein 3
• MAS1 protein, human
• Proto-Oncogene Mas
• ATP2C1 protein, human
• Calcium-Transporting ATPases

Topics

• Animals
• Animals, Newborn
• Autoantibodies (blood, immunology, isolation & purification)
• Calcium-Transporting ATPases (immunology)
• Chromatography, Affinity (methods)
• Desmoglein 1 (immunology)
• Desmoglein 3 (immunology)
• Humans
• Keratinocytes (enzymology, metabolism)
• Mice
• Mice, Inbred BALB C
• Pemphigus (immunology, pathology)
• Proto-Oncogene Mas