Integrated landscape of copy number variation and RNA expression associated with nodal metastasis in invasive ductal breast carcinoma.

Abstract

BACKGROUND:

Lymph node metastasis (NM) in breast cancer is a clinical predictor of patient outcomes, but how its genetic underpinnings contribute to aggressive phenotypes is unclear. Our objective was to create the first landscape analysis of CNV-associated NM in ductal breast cancer. To assess the role of copy number variations (CNVs) in NM, we compared CNVs and/or associated mRNA expression in primary tumors of patients with NM to those without metastasis.

RESULTS:

We found CNV loss in chromosomes 1, 3, 9, 18, and 19 and gains in chromosomes 5, 8, 12, 14, 16-17, and 20 that were associated with NM and replicated in both databases. In primary tumors, per-gene CNVs associated with NM were ten times more frequent than mRNA expression; however, there were few CNV-driven changes in mRNA expression that differed by nodal status. Overlapping regions of CNV changes and mRNA expression were evident for the CTAGE5 gene. In 8q12, 11q13-14, 20q1, and 17q14-24 regions, there were gene-specific gains in CNV-driven mRNA expression associated with NM.

METHODS:

Data on CNV and mRNA expression from the TCGA and the METABRIC consortium of breast ductal carcinoma were utilized to identify CNV-based features associated with NM. Within each dataset, associations were compared across omic platforms to identify CNV-driven variations in gene expression. Only replications across both datasets were considered as determinants of NM.

CONCLUSIONS:

Gains in CTAGE5, NDUFC2, EIF4EBP1, and PSCA genes and their expression may aid in early diagnosis of metastatic breast carcinoma and have potential as therapeutic targets.

• Authors: Michael Behring, Sadeep Shrestha, Upender Manne, Xiangqin Cui, Agustin Gonzalez-Reymundez, Alexander Grueneberg, Ana I Vazquez
• Journal: Oncotarget (Oncotarget) Vol. 9 Issue 96 Pg. 36836-36848 (Dec 07 2018) ISSN: 1949-2553 [Electronic] United States
• PMID: 30627325 (Publication Type: Journal Article)