Interleukin-6 promotor gene polymorphisms and susceptibility to chronic hepatitis B virus in Egyptians.

Abstract

AIM:

To investigate the association between IL-6 polymorphisms (-174G/C, -572G/C and -597G/A) and susceptibility to chronic hepatitis B virus (CHB) infection.

METHOD:

Total 108 subjects with CHB infection and 102 healthy controls were enrolled in this study. IL-6 (-174G/C) was genotyped using Mutagenically separated Polymerase Chain Reaction (MS-PCR) while sequence specific primers-PCR (SSP-PCR) was used for studying -572G/C and -597G/A. IL-6 plasma level was measured using Enzyme-linked immunosorbent assay (ELISA).

RESULTS:

A significant increase (P < 0.01, P < 0.01, P < 0.001) in -174GG, -572GC and -597GA; respectively in the CHB group compared to control group, while -572GG genotype was significantly decreased (P < 0.01) in CHB patients. A significant increase (p < 0.01, p < 0.01) in -174 G and -597A alleles was observed in the CHB patient group; respectively. GGA haplotype is significantly increased (P < 0.05) while GCA haplotype is significantly decreased (P < 0.001) in the patient group. A moderate linkage disequilibrium (LD) (D' = 0.719, r2 = 0.474; P < 0.001) between IL-6 (-572G/C and -597G/A) was observed. A significant reduction (P < 0.01) in IL-6 plasma level in CHB patients compared to healthy controls (22.28 ± 1.93 versus 32.08 ± 2.41), which was negatively correlated (r = -0.216; P < 0.01) with HBV infection.

CONCLUSIONS:

This study pointed to the potential role of IL-6 (-174G/C, -572 G/C and -597G/A) gene polymorphisms in the susceptibility to HBV infection. Our results allow for only preliminary conclusions due to relatively small sample size. There is a need for further larger scale studies to fully examine the possible relationship between these cytokine gene polymorphisms and the development of CHB.

• Authors: Eman A El-Maadawy, Roba M Talaat, Maha M Ahmed, Soha Z El-Shenawy
• Journal: Human immunology (Hum Immunol) Vol. 80 Issue 3 Pg. 208-214 (Mar 2019) ISSN: 1879-1166 [Electronic] United States
• PMID: 30594561 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier Inc.

Chemical References

• Biomarkers
• Interleukin-6

Topics

• Adult
• Alleles
• Biomarkers
• Egypt
• Female
• Gene Frequency
• Genetic Predisposition to Disease
• Genotype
• Haplotypes
• Hepatitis B virus
• Hepatitis B, Chronic (blood, etiology)
• Humans
• Interleukin-6 (blood, genetics)
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Promoter Regions, Genetic