US10 Protein Is Crucial but not Indispensable for Duck Enteritis Virus Infection in Vitro.

Abstract

To investigate the function of the duck enteritis virus (DEV) tegument protein US10, we generated US10 deletion and revertant mutants (ΔUS10 and US10FRT) via two-step RED recombination based on an infectious BAC clone of DEV CHv-BAC-G (BAC-G). In multistep growth kinetic analyses, ΔUS10 showed an approximately 100-fold reduction in viral titer, while the genome copies decreased only 4-fold compared to those of BAC-G. In one-step growth kinetic analyses, there were no significant differences in genome copies among BAC-G, ΔUS10 and US10FRT, but ΔUS10 still showed a 5- to 20-fold reduction in viral titer, and the replication defect of ΔUS10 was partially reversed by infection of US10-expressing cells. The transcription levels of Mx, OASL, IL-4, IL-6 and IL-10 in ΔUS10-infected duck embryo fibroblasts (DEFs) were significantly upregulated, while TLR3 was downregulated compared with those in BAC-G-infected DEFs. Taken together, these data indicated that US10 is vital for DEV replication and is associated with transcription of some immunity genes.

• Authors: Yunchao Ma, Qiurui Zeng, Mingshu Wang, Anchun Cheng, Renyong Jia, Qiao Yang, Ying Wu, Xin-Xin Zhao, Mafeng Liu, Dekang Zhu, Shun Chen, Shaqiu Zhang, Yunya Liu, Yanling Yu, Ling Zhang, Xiaoyue Chen
• Journal: Scientific reports (Sci Rep) Vol. 8 Issue 1 Pg. 16510 (11 07 2018) ISSN: 2045-2322 [Electronic] England
• PMID: 30405139 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Viral Proteins

Topics

• Animals
• Bird Diseases (immunology, virology)
• Cell Line
• Ducks (virology)
• Enteritis (veterinary)
• Gene Deletion
• Gene Expression Regulation, Viral
• Host-Pathogen Interactions (immunology)
• Mardivirus (genetics, immunology, metabolism, pathogenicity)
• Open Reading Frames
• Viral Proteins (genetics, metabolism)
• Virus Replication (genetics)