Diagnostic accuracy of glypican-3 in differentiating hepatocellular carcinoma from metastatic liver tumours.

Abstract

OBJECTIVE:

To determine the diagnostic accuracy of Glypican-3 in differentiating hepatocellular carcinoma from metastatic liver tumours while taking histopathology as the gold standard..

METHODS:

The cross-sectional study was conducted at Shaukat Khanum Memorial Cancer Hospital, Lahore, Pakistan, from January 1 to June 30, 2016, and comprised cases of malignant liver tumours. Samples were collected from the pathology department. Glypican-3was applied on them. Tumours were classified as positive when they showed >5% positivity and negative when showing <5% positivity. Frequencies and percentages of cases showing GPC3 positivity and negativity along with frequency and percentages of hepatocellular carcinoma and metastatic tumours were calculated.

RESULTS:

Of the 240 patients, 143(59.58%) were males and 97 (40.42%) were females. Overall mean age was 54.65 ± 13.46 years. On histopathology, 134 cases were hepatocellular carcinoma (55.83%) and 106 (44. 17%) cases turned out to be metastatic carcinoma. Glypican-3staining was positive in 116 (48.33%) cases and negative in 124(51.67%). Sensitivity was 82%, Specificity 94.33%, positive predictive value 94.82% and negative predictive value 80.64%. Diagnostic accuracy was 87.5%.

CONCLUSIONS:

Glypican-3 was found to be a highly sensitive and specific Immunohistochemistry stain distinguishing hepatocellular carcinoma from the clear majority of metastatic carcinomas of the liver.

• Authors: Sadia Majeed, Sajid Mushtaq, Muhammad Azam, Noreen Akhtar, Mudassar Hussain, Asif Loya
• Journal: JPMA. The Journal of the Pakistan Medical Association (J Pak Med Assoc) Vol. 68 Issue 7 Pg. 1029-1031 (Jul 2018) ISSN: 0030-9982 [Print] Pakistan
• PMID: 30317296 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• GPC3 protein, human
• Glypicans

Topics

• Adolescent
• Adult
• Aged
• Biomarkers, Tumor (metabolism)
• Biopsy, Fine-Needle
• Carcinoma, Hepatocellular (diagnosis, metabolism)
• Cross-Sectional Studies
• Diagnosis, Differential
• Female
• Glypicans (metabolism)
• Humans
• Liver (metabolism, pathology)
• Liver Neoplasms (diagnosis, metabolism)
• Male
• Middle Aged
• Neoplasm Metastasis (diagnosis)
• Reproducibility of Results
• Retrospective Studies
• Young Adult