Review of Chlamydia trachomatis viability methods: assessing the clinical diagnostic impact of NAAT positive results.

Abstract

INTRODUCTION:

Chlamydia trachomatis (chlamydia) is the most commonly diagnosed bacterial sexually transmitted infection (STI) worldwide. The advancement of molecular techniques has made chlamydia diagnostics infinitely easier. However, molecular techniques lack the information on chlamydia viability. Where in routine diagnostics the detection of chlamydia DNA or RNA might suffice, in other patient scenarios, information on the viability of chlamydia might be essential. Areas covered: In this review, the authors discuss the specific strengths and limitations of currently available methods to evaluate chlamydia viability: conventional cell culture, messenger RNA (mRNA) detection and viability-PCR (V-PCR). PubMed and Google Scholar were searched with the following terms: Chlamydia trachomatis, Treatment failure, Anal chlamydia, Microbial viability, Culture, Viability-PCR, Messenger RNA, and Molecular diagnostics Expert commentary: Several techniques are currently available to determine chlamydia viability and thus the clinical relevance of a positive test result in clinical samples. Depending on the underlying research question, all three discussed techniques have their merits when testing for viability. However, mRNA methods show the most promise in determining the presence of a true infection, in case the chlamydia reticulate body can be specifically detected. Further research is needed to understand how to best apply viability testing in current chlamydia diagnostics.

• Authors: Kevin J H Janssen, Jeanne A M C Dirks, Nicole H T M Dukers-Muijrers, Christian J P A Hoebe, Petra F G Wolffs
• Journal: Expert review of molecular diagnostics (Expert Rev Mol Diagn) Vol. 18 Issue 8 Pg. 739-747 (08 2018) ISSN: 1744-8352 [Electronic] England
• PMID: 29987959 (Publication Type: Journal Article, Systematic Review)

Topics

• Bacteriological Techniques (methods)
• Cell Survival
• Chlamydia Infections (diagnosis, microbiology)
• Chlamydia trachomatis (genetics, isolation & purification, pathogenicity)
• Humans
• Molecular Diagnostic Techniques (methods)
• Polymerase Chain Reaction (methods)
• Transcriptome