Cancer-Cell Imaging Using Copper-Doped Zeolite Imidazole Framework-8 Nanocrystals Exhibiting Oxidative Catalytic Activity.

Abstract

Copper-doped zeolite imidazole framework-8 (Cu/ZIF-8) was prepared and its peroxidase-like oxidative catalytic activity was examined with a demonstration of its applicability for cancer-cell imaging. Through simple solution chemistry at room temperature, Cu/ZIF-8 nanocrystals were produced that catalytically oxidized an organic substrate of o-phenylenediamine in the presence of H2 O2 . In a similar manner to peroxidase, the Cu/ZIF-8 nanocrystals oxidized the substrate through a ping-pong mechanism with an activation energy of 59.2 kJ mol-1 . The doped Cu atoms functioned as active sites in which the active Cu intermediates were expected to be generated during the catalysis, whereas the undoped ZIF-8 did not show any oxidative activity. Cu/ZIF-8 nanocrystals exhibited low cell toxicity and displayed catalytic activity through interaction with H2 O2 among various reactive oxygen species in a cancer cell. This oxidative activity in vitro allowed cancer-cell imaging by exploiting the photoluminescence emitted from the oxidized product of o-phenylenediamine, which was insignificant in the absence of the Cu/ZIF-8 nanocrystals. The results of this study suggest that the Cu/ZIF-8 nanocrystal is a promising catalyst for the analysis of the microbiological systems.

• Authors: Changjoon Keum, Sangwoo Park, Sang-Yup Lee
• Journal: Chemistry, an Asian journal (Chem Asian J) Vol. 13 Issue 18 Pg. 2641-2648 (Sep 17 2018) ISSN: 1861-471X [Electronic] Germany
• PMID: 29920956 (Publication Type: Journal Article)
• Copyright: © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Imidazoles
• Phenylenediamines
• Zeolites
• Copper
• 1,2-diaminobenzene
• Hydrogen Peroxide
• Peroxidase

Topics

• Biomimetic Materials (chemistry, toxicity)
• Catalysis
• Copper (chemistry, toxicity)
• HEK293 Cells
• HeLa Cells
• Humans
• Hydrogen Peroxide (chemistry)
• Imidazoles (chemistry, toxicity)
• Kinetics
• Microscopy, Confocal (methods)
• Microscopy, Fluorescence (methods)
• Nanoparticles (chemistry, toxicity)
• Neoplasms (diagnosis)
• Oxidation-Reduction
• Particle Size
• Peroxidase (chemistry)
• Phenylenediamines (chemistry)
• Porosity
• Zeolites (chemistry, toxicity)