Immunogenicity and protective capacity of EF-Tu and FtsZ of Streptococcus suis serotype 2 against lethal infection.

Abstract

Vaccine development efforts against Streptococcus suis serotype 2 (S. suis 2) are often constrained by strain/serotype antigen variability. Bioinformatics analyses revealed two highly conserved S. suis 2 factors, EF-Tu and FtsZ. Murine immunization with recombinant proteins emulsified in white oil adjuvant or eukaryotic DNA vaccine vectors provided significant protection against lethal S. suis 2 challenge. Immune responses elicited by recombinant protein immunization revealed the robust generation of humoral immune responses, with a mixed induction of Th1-type and Th2-type responses. Furthermore, the antiserum from mice immunized with recombinant proteins significantly inhibited the growth of S. suis 2 in healthy pig whole blood, suggesting the triggering of a strong opsonizing response. Histological examination found that immunizing mice with purified recombinant proteins reduced neutrophil and macrophage accumulation in brain and lung tissues after challenge with virulent S. suis. Taken together, these findings reveal that EF-Tu and FtsZ may be promising targets for subunit and DNA vaccine candidates against S. suis 2 infection.

• Authors: Liping Feng, Xiaona Niu, Wen Mei, Weitian Li, Yuan Liu, Stephan P Willias, Chao Yuan, Weicheng Bei, Xiaohong Wang, Jinquan Li
• Journal: Vaccine (Vaccine) Vol. 36 Issue 19 Pg. 2581-2588 (05 03 2018) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 29627237 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• Bacterial Proteins
• Bacterial Vaccines
• Cytoskeletal Proteins
• FtsZ protein, Bacteria
• Vaccines, DNA
• Peptide Elongation Factor Tu

Topics

• Animals
• Bacterial Proteins (genetics, immunology)
• Bacterial Vaccines (genetics, immunology, pharmacology)
• Cytoskeletal Proteins (genetics, immunology)
• Disease Models, Animal
• Female
• Mice, Inbred BALB C
• Peptide Elongation Factor Tu (genetics, immunology)
• Streptococcal Infections (etiology, prevention & control)
• Streptococcus suis (classification, pathogenicity)
• Th1 Cells (immunology)
• Th2 Cells (immunology)
• Vaccines, DNA (immunology, pharmacology)