Anti-Cancer Effect of α-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells.

Abstract

BACKGROUND:

α-Solanine, the most important and active component of Solanum nigrum, was found to have anti-cancer activity on multiple cancer cells. However, its effects on colorectal cancer (CRC) and associated molecular mechanisms remain to be further elucidated.

OBJECTIVE:

In this study, we investigated the anti-cancer effects of α-solanine against CRC cells in vitro and in vivo.

MATERIALS & METHODS:

Cell viability was measured using Cell Counting Kit-8 (CCK-8) assay; cell cycle was analyzed with a Cycletest Plus DNA Reagent Kit; cell apoptosis was detected by flow cytometer; cell migration and invasive ability was determined by Transwell assays; S100P protein expression was also analyzed by western blotting; lentiviral vectors expressing shRNA targeting the S100P gene.

RESULTS:

We demonstrated that α-solanine inhibited CRC cell (SW480, SW620 and HT-29) growth as well as migration and invasion, induced cell cycle arrest and apoptosis in vitro, and suppressed tumor growth in vivo. Moreover, we observed that S100P expression was downregulated by α-solanine. Overexpression of S100P partially reversed the α-solanine-induced growth inhibition of CRC cells. Conversely, knockdown of S100P by lentiviral-mediated RNAi resulted in significantly promoting the α-solanine-induced growth inhibition.

CONCLUSION:

These findings suggest that α-solanine is a potential agent for the treatment of CRC, and the anti-tumor effect of α-solanine in the CRC cells may be mediated at least partly by the downregulation of S100P.

• Authors: Xiaofen Ni, Jiajun Chen, Fengying Lu, Zhengzhong Yuan, Xiaofeng Xu, Zhen Hu, Lei Jiang
• Journal: Recent patents on anti-cancer drug discovery (Recent Pat Anticancer Drug Discov) Vol. 13 Issue 2 Pg. 240-247 ( 2018) ISSN: 2212-3970 [Electronic] United Arab Emirates
• PMID: 29600769 (Publication Type: Journal Article)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• Antineoplastic Agents
• Calcium-Binding Proteins
• Neoplasm Proteins
• S100P protein, human
• alpha-solanine
• Solanine

Topics

• Animals
• Antineoplastic Agents (pharmacology, therapeutic use)
• Calcium-Binding Proteins (antagonists & inhibitors, biosynthesis, genetics)
• Cell Line, Tumor
• Colorectal Neoplasms (drug therapy, metabolism)
• Down-Regulation (drug effects, physiology)
• Gene Expression Regulation, Neoplastic (drug effects)
• HT29 Cells
• Humans
• Mice
• Mice, Nude
• Neoplasm Proteins (antagonists & inhibitors, biosynthesis, genetics)
• Solanine (pharmacology, therapeutic use)
• Treatment Outcome
• Tumor Burden (drug effects, physiology)
• Xenograft Model Antitumor Assays (methods)