Abstract | BACKGROUND: METHODS: A ciprofloxacin-eluting sinus stent was created by coating ciprofloxacin/ Eudragit RS100 on biodegradable poly-D/L-lactic acid (2 mg). After analyzing in-vitro inhibition of P aeruginosa (PAO-1 strain) biofilm formation, a total of 8 stents (4 shams, 4 CSSs) were placed unilaterally in rabbit maxillary sinuses via dorsal sinusotomy after inducing infection for 1 week with PAO-1. Animals were assessed 2 weeks after stent insertion with nasal endoscopy, sinus culture, computed tomography (CT) scan, histopathology, and scanning electron microscopy (SEM). RESULTS: PAO-1 biofilm formation was significantly reduced in vitro with exposure to the CSS (p < 0.0001). Insertion of the stent in PAO-1-infected rabbits for 2 weeks resulted in significant improvement in sinusitis according to endoscopy scoring (p < 0.0001) and CT scoring (p < 0.002). Histology and SEM revealed marked improvement in the structure of the mucosa and submucosa with no detection of biofilm structures in the CSS cohort. CONCLUSION: Although this study had a small sample size, we identified robust therapeutic efficacy of the CSS by reducing bacterial load and biofilm formation of P aeruginosa in a preclinical model of sinusitis after placement for 2 weeks.
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Authors | Do-Yeon Cho, Dong-Jin Lim, Calvin Mackey, Daniel Skinner, Christopher Weeks, Gobind S Gill, Robert W Hergenrother, William E Swords, Bradford A Woodworth |
Journal | International forum of allergy & rhinology
(Int Forum Allergy Rhinol)
Vol. 8
Issue 4
Pg. 482-489
(04 2018)
ISSN: 2042-6984 [Electronic] United States |
PMID | 29334430
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2018 ARS-AAOA, LLC. |
Chemical References |
- Anti-Bacterial Agents
- Ciprofloxacin
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Topics |
- Animals
- Anti-Bacterial Agents
(therapeutic use)
- Bacterial Load
- Biofilms
(drug effects, growth & development)
- Cells, Cultured
- Ciprofloxacin
(therapeutic use)
- Disease Models, Animal
- Drug-Eluting Stents
- Endoscopy
- Humans
- Maxillary Sinus
(drug effects, surgery)
- Nasal Mucosa
(pathology)
- Pseudomonas Infections
(therapy)
- Pseudomonas aeruginosa
(physiology)
- Rabbits
- Sinusitis
(therapy)
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