HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Aspergillus fumigatus-sensitive IgE is associated with bronchial hypersensitivity in a murine model of neutrophilic airway inflammation.

Abstract
Neutrophils are the predominant inflammatory cells that infiltrate airways during acute exacerbation of asthma. The importance of A. fumigatus sensitization, and IgE response in the airways in patients with acute asthma is unclear. Rockefeller (RK) mice were sensitized with A. fumigatus extract protein. The animals were subsequently challenged with different degrees of A. fumigatus contamination in the cage bedding. All groups of mice were euthanized to obtain bronchoalveolar lavage fluid (BALF) for cytological and Elisa assays, and lung tissue for histological analysis. Moreover, several bioassays were conducted to determine whether BALF IgE antibodies can activate mast cells. In this study, we demonstrated that exposure of sensitized mice to a known concentration of A. fumigatus conidia produces bronchial hyperreactivity with marked neutrophilic bronchial infiltration and increased BALF IgE, capable of triggering mast cell degranulation. This study suggests that IgE may play a role in bronchial hyperreactivity associated to A. fumigatus exposure in mice. Mice sensitized and challenged with this fungus showed characteristics of severe asthma, with an increase of BALF neutrophils, histological changes consistent with severe asthma and an increase of IgE capable of triggering type I hypersensitivity.
AuthorsG Morán, B Uberti, A Ortloff, H Folch
JournalJournal de mycologie medicale (J Mycol Med) Vol. 28 Issue 1 Pg. 128-136 (Mar 2018) ISSN: 1773-0449 [Electronic] France
PMID29233467 (Publication Type: Journal Article)
CopyrightCopyright © 2017 Elsevier Masson SAS. All rights reserved.
Chemical References
  • Fungal Proteins
  • Immunoglobulin E
Topics
  • Animals
  • Aspergillus fumigatus (chemistry, immunology)
  • Asthma (immunology)
  • Biological Assay
  • Bronchial Hyperreactivity (immunology, microbiology, physiopathology)
  • Bronchoalveolar Lavage Fluid (immunology)
  • Disease Models, Animal
  • Female
  • Fungal Proteins (administration & dosage, immunology)
  • Hypersensitivity
  • Immunoglobulin E (immunology)
  • Inflammation
  • Lung (microbiology, pathology)
  • Mast Cells (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils (immunology)
  • Spores, Fungal (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: