Abstract | OBJECTIVE: To examine specific molecular mechanisms involved in modulating hepatic lipogenesis and mitochondria biogenesis signals by Lithospermum erythrorhizon (gromwell) root extract. METHODS: RESULTS: Gromwell root extract and its naphthoquinones dose-dependently repressed high glucose and liver X receptor α induction of SREBP1c promoter activity and gene expression. Hepatic lipogenesis was repressed, and PGC1α promoter and gene expression and ERRα response element activity were increased by gromwell root extract. Gromwell root extract, shikonin, and α-methyl-n-butyrylshikonin increased AMPKα phosphorylation, and inhibition of AMPK blunted the repression in SREBP1c promoter activity by gromwell root extract and its naphthoquinones. CONCLUSIONS: Data suggest that gromwell root extract and its naphthoquinones repress lipogenesis by increasing the phosphorylated state of AMPKα and stimulating mitochondrial biogenesis signals.
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Authors | Rodney A Velliquette, Arun Rajgopal, John Rebhun, Kelly Glynn |
Journal | Obesity (Silver Spring, Md.)
(Obesity (Silver Spring))
Vol. 26
Issue 1
Pg. 126-134
(01 2018)
ISSN: 1930-739X [Electronic] United States |
PMID | 29165897
(Publication Type: Journal Article)
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Copyright | © 2017 The Obesity Society. |
Chemical References |
- Naphthoquinones
- Sterol Regulatory Element Binding Protein 1
- shikonin
- AMP-Activated Protein Kinases
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Topics |
- AMP-Activated Protein Kinases
(metabolism)
- Animals
- CHO Cells
- Cricetulus
- Hep G2 Cells
- Humans
- Lithospermum
(chemistry)
- Naphthoquinones
(chemistry)
- Sterol Regulatory Element Binding Protein 1
(metabolism)
- Transfection
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