Recombinant latcripin 11 of Lentinula edodes C91-3 suppresses the proliferation of various cancer cells.

Abstract

Lentinula edodes C91-3 is an edible mushroom that has demonstrated a remarkable anti-tumor effect in various cancer cells both in vitro and in vivo. In the present study, we report the ability of recombinant thioredoxin-like latcripin 11 (LP-11) of Lentinula edodes C91-3 to suppress the proliferation of various cancer cells. The LP-11 gene of Lentinula edodes C91-3 was cloned in the pET-32a(+) expression vector and expressed in a prokaryotic system. The expressed protein was refolded by gradual dialysis and purified by affinity gel filtration chromatography. The antioxidant activity of LP-11 was tested by 1,1-dipheny l-2-picrylhydrazyl (DPPH) assay. The anti-tumor activity of recombinant LP-11 was tested in eight kinds of tumor cell lines by CCK-8 assay. Recombinant LP-11 significantly suppressed the proliferation of various cancer cells, but not normal human umbilical vein endothelial cells. Human lymphoma U937 cells exhibited the most sensitivity to LP-11 protein. U937 cell apoptosis was assessed by Annexin V staining coupled with flow cytometry, and mitochondrial morphology was analyzed by light and electron microscopy. It was revealed that recombinant LP-11 induced apoptosis in human leukemic monocyte lymphoma U937 cells. Our findings suggest that recombinant LP-11 is a promising agent for the treatment of lymphoma.

• Authors: Yifan Gao, Arshad Ahmed Padhiar, Jia Wang, Wei Zhang, Mintao Zhong, Ben Liu, Zhijie Kang, Xiaoli Wang, Xingyun Li, Min Huang
• Journal: Gene (Gene) Vol. 642 Pg. 212-219 (Feb 05 2018) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 29111208 (Publication Type: Journal Article)
• Copyright: Copyright © 2017. Published by Elsevier B.V.

Chemical References

• Antineoplastic Agents, Phytogenic
• Antioxidants
• Fungal Proteins
• Recombinant Proteins

Topics

• A549 Cells
• Antineoplastic Agents, Phytogenic (pharmacology)
• Antioxidants (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Drug Screening Assays, Antitumor
• Fungal Proteins (genetics, pharmacology)
• HL-60 Cells
• Hep G2 Cells
• Human Umbilical Vein Endothelial Cells
• Humans
• K562 Cells
• Mitochondria (drug effects)
• Neoplasms (drug therapy, metabolism)
• Recombinant Proteins (pharmacology)
• Shiitake Mushrooms (metabolism)
• U937 Cells