Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis.

Abstract

Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

• Authors: Konstantin P Lyashchenko, Adrian Grandison, Karen Keskinen, Alina Sikar-Gang, Paul Lambotte, Javan Esfandiari, Gregory C Ireton, Aarthy Vallur, Steven G Reed, Gareth Jones, H Martin Vordermeier, Judy R Stabel, Tyler C Thacker, Mitchell V Palmer, W Ray Waters
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 24 Issue 12 (Dec 2017) ISSN: 1556-679X [Electronic] United States
• PMID: 28978510 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 American Society for Microbiology.

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial

Topics

• Animals
• Antibodies, Bacterial (blood)
• Antigens, Bacterial (immunology)
• Cattle
• Immunoassay (methods)
• Mycobacterium bovis (immunology)
• Serologic Tests (methods)
• Tuberculosis, Bovine (immunology)