CAY10591, a SIRT1 activator, suppresses cell growth, invasion, and migration in gingival epithelial carcinoma cells.

Abstract

SIRT1 is a NAD-dependent histone deacetylase that is important in a wide variety of physiological and pathophysiological processes. Although many studies have examined the relationship between SIRT1 and cancer, the role of SIRT1 in tumor malignancy is controversial. Here, we examined the effects of the SIRT1 activator CAY10591 in gingival epithelial carcinoma Ca9-22 cells. CAY10591 treatment dose- and time-dependently increased SIRT1 level and activity. The treatment decreased cell growth and induced cell-cycle repressor p21 levels. In addition, dimethyl sulfoxide significantly reduced cellular invasion and migration, and CAY10591 enhanced this decrease. Quantitative PCR analysis showed that CAY10591 decreased expression of several invasion/migration promoter genes and induced repressor genes. Our findings suggest that CAY10591 suppresses cell growth and invasion/migration activity in gingival squamous cell carcinoma Ca9-22 cells.

• Authors: Takahisa Murofushi, Hiromasa Tsuda, Yoshikazu Mikami, Yoko Yamaguchi, Naoto Suzuki
• Journal: Journal of oral science (J Oral Sci) Vol. 59 Issue 3 Pg. 415-423 ( 2017) ISSN: 1880-4926 [Electronic] Japan
• PMID: 28904318 (Publication Type: Journal Article)

Chemical References

• CAY10591
• Cyclopentanes
• Pyrroles
• Quinoxalines
• SIRT1 protein, human
• Sirtuin 1
• Dimethyl Sulfoxide

Topics

• Cell Division (drug effects)
• Cell Line, Tumor
• Cyclopentanes (pharmacology)
• Dimethyl Sulfoxide (pharmacology)
• Gingival Neoplasms (pathology)
• Humans
• Neoplasm Invasiveness (genetics, prevention & control)
• Neoplasm Metastasis (genetics, prevention & control)
• Pyrroles (pharmacology)
• Quinoxalines (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Sirtuin 1 (metabolism)