Leptocarpin Suppresses Proliferation, Migration, and Invasion of Human Osteosarcoma by Targeting Type-1 Insulin-Like Growth Factor Receptor (IGF-1R).

Abstract

BACKGROUND Leptocarpin (LTC) has drawn much attention for suppressing tumor growth or reducing inflammation. However, the effect of LTC on osteosarcoma has rarely been reported. Our object was to determine whether LTC suppresses MG63 cell proliferation, migration, and invasion, and whether type-1 insulin-like growth factor receptor (IGF-1R) is one of the targets in LTC suppressing osteosarcoma. MATERIAL AND METHODS Cytotoxicity of LTC was performed by use of a cell-counting kit-8 (CCK-8). RNA interference (RNAi) or pEABE-bleo IGF-1R plasmid were used for silencing or overexpressing IGF-1R, Western blot (WB) analysis was used for IGF-1R expression, CCK-8 for proliferation, and transwell assay for migration and invasion. RESULTS LTC (23.533 μM) treatment for 48 h was taken as the 50% inhibiting concentration (IC50), which significantly (P<0.05) suppressed MG63 cells proliferation, migration, and invasion. LTC (IC50) obviously inhibited IGF-1R expression in MG63 cells, with similar effect to small interfering RNA (siRNA), while pEABE-bleo IGF-1R transfection overexpressed IGF-1R. siRNA silencing IGF-1R suppressed MG63 cells proliferation, migration, and invasion, while pEABE-bleo IGF-1R transfection was significantly (P<0.05) promoted. With or without siRNA or pEABE-bleo IGF-1R transfection, LTC (IC50) suppressed MG63 cells proliferation, migration, and invasion. The effect of LTC (IC50) combined with siRNA on suppressing MG63 cells proliferation, migration, and invasion was more obvious, while the effect of LTC (IC50) combined with pEABE-bleo IGF-1R transfection was less significant (P<0.05). CONCLUSIONS LTC suppressed osteosarcoma proliferation, migration, and invasion by inhibiting IGF-1R expression. IGF-1R is one of the targets in LTC suppressing osteosarcoma.

• Authors: Shao-Wei Zheng, Wen-Guo Wan, Hai-Xiong Miao, Rui Tang, Bin Wang, Qi-Zhi Huang, Wei-le Liu, Jian-Ping Zheng, Chu-Qun Chen, Hao-Bo Zhong, Sheng-Fa Li, Chun-Han Sun
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 23 Pg. 4132-4140 (Aug 27 2017) ISSN: 1643-3750 [Electronic] United States
• PMID: 28844074 (Publication Type: Journal Article)

Chemical References

• IGF1R protein, human
• MicroRNAs
• RNA, Small Interfering
• Receptors, Somatomedin
• Sesquiterpenes
• leptocarpin
• Receptor, IGF Type 1

Topics

• Apoptosis (drug effects)
• Bone Neoplasms (drug therapy, genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Down-Regulation (drug effects)
• Humans
• MicroRNAs (genetics, metabolism)
• Osteosarcoma (drug therapy, genetics, metabolism, pathology)
• RNA Interference
• RNA, Small Interfering (genetics)
• Receptor, IGF Type 1
• Receptors, Somatomedin (antagonists & inhibitors, metabolism)
• Sesquiterpenes (pharmacology)
• Signal Transduction