Abstract | OBJECTIVES:
Taraxerol acetate has potent anti- cancer effects via the induction of apoptosis, autophagy, cell cycle arrest, and inhibition of cell migration. However, whether taraxerol induced apoptosis and its underlying mechanisms of action is not clear. In the present study, we assess the effects of taraxerol on the mitochondrial apoptotic pathway and determine the release of cytochrome c to the cytosol and activation of caspases. MATERIALS AND METHODS: In this experimental study, we mainly investigated the effect of taraxerol on HeLa cells. We tested cell viability by the MTT assay and morphologic changes, analyzed apoptosis by DAPI staining and flow cytometry. We also determined reactive oxygen species (ROS) and mitochondrial membrane potential ( MMP) using a Microplate Reader. In addition, the apoptotic proteins were tested by Western blot. RESULTS: CONCLUSIONS: These results demonstrated that taraxerol induced cell apoptosis through a mitochondria-mediated pathway in HeLa cells. Thus, taraxerol might be a potential anticervical cancer candidate.
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Authors | Xiangyang Yaoi, Binyu Lu, Chaotian Lü, Qin Bai, Dazhong Yan, Hui Xu |
Journal | Cell journal
(Cell J)
Vol. 19
Issue 3
Pg. 512-519
(Oct 2017)
ISSN: 2228-5806 [Print] Iran |
PMID | 28836414
(Publication Type: Journal Article)
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